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Antigenic polypeptides capable of identifying Trxc antibodies and application of antigenic polypeptides

An antibody recognition, antigen polypeptide technology, applied in the field of biomedicine, can solve the problems of high production cost, reduced specificity, increased false positives, etc., and achieves the effects of low cost, simple preparation and high detection efficiency

Inactive Publication Date: 2016-10-12
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The antigen used by IGRA is the whole protein encoded by the RD (region of difference) gene of Mycobacterium tuberculosis, which is expensive to produce and is not recommended by the World Health Organization as a screening method for low- and middle-income countries.
And some studies have also shown that in countries with high prevalence of tuberculosis, the diagnosis of IGRA is interfered by environmental factors, the specificity is reduced, and the false positives are increased.
[0005] Therefore, the current research on tuberculosis serum immunological diagnosis and detection still needs to be strengthened

Method used

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  • Antigenic polypeptides capable of identifying Trxc antibodies and application of antigenic polypeptides
  • Antigenic polypeptides capable of identifying Trxc antibodies and application of antigenic polypeptides
  • Antigenic polypeptides capable of identifying Trxc antibodies and application of antigenic polypeptides

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Embodiment 1

[0065] 1. Preparation of polypeptides

[0066] 1. Preparation of Peptide Library

[0067] The amino acid sequence of Trxc was obtained from the protein public resource database uni-prot.

[0068] 2. In situ synthesis of Trxc protein peptide library to prepare peptide array

[0069] According to the length of protein and peptide amino acid residues and the length of overlapping sequences, the peptide synthesizer ASP SL (Germany, intavis company) MutilPep synthesis control program is set, and a specific amino acid is added to the activated nitrocellulose in each synthesis cycle according to the program control Synthetic site on membrane surfaces and catalyzes peptide bond formation between amino acids. After the peptide chip was synthesized, it was sealed and stored at -20°C for future use.

[0070] 2. Screening of peptides related to tuberculosis (screening of antigenic epitopes)

[0071] 1. Hydration and Western Blotting of Peptide Chips

[0072]Immerse the peptide chip p...

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Abstract

The invention discloses antigenic polypeptides capable of identifying Trxc antibodies and an application of the antigenic polypeptides. Polypeptide active fragments of the antigenic polypeptides are at least one of following sequences: 1), a sequence represented as SEQ ID NO:1: SFATDVLSSNKPVLV; 2), an amino acid derivative sequence formed by the amino acid sequence represented as SEQ ID NO:1 through amino acid residue modification, substitution, deletion or addition. The antigenic polypeptides capable of identifying the Trxc antibodies can effectively detect the Trxc antibody level in a sample such as a blood sample (for example, a serum sample) of a tuberculosis patient or a suspected tuberculosis patient.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, the present invention relates to antigenic polypeptides recognized by Trxc antibodies and uses thereof. More specifically, the present invention relates to an antigenic polypeptide recognized by the Trxc antibody, the use of the antigenic polypeptide in preparing a kit for detecting the level of the Trxc antibody in serum, and a kit for detecting the level of the Trxc antibody in a sample. Background technique [0002] Tuberculosis is one of the three major infectious diseases in the world. The epidemiological investigation report released by the World Health Organization (WTO) shows that the pathology of Mycobacterium tuberculosis infection in my country accounts for 12% of the total number of cases in the world, ranking second in the world after India . The high incidence and prevalence of tuberculosis have seriously affected the social and economic development of our country. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/35G01N33/569
CPCC07K14/35G01N33/5695G01N2333/35G01N2469/20
Inventor 岳文涛谭金晶顾勐张丽娜
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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