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Preparation of three-dimensional metal-enhanced fluorescence/colorimetric dual-mode paper chip and ATP detection

A metal-enhanced fluorescence, chip detection technology, applied in the direction of determination/inspection of microorganisms, measurement devices, biochemical equipment and methods, etc., can solve the problems of limited application, poor fluorescence effect signal, etc. , the effect of accurate fluorescence measurement

Inactive Publication Date: 2018-06-08
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the strong background fluorescence of the paper fiber substrate, the fluorescence effect signal is poor, which limits its further application.

Method used

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  • Preparation of three-dimensional metal-enhanced fluorescence/colorimetric dual-mode paper chip and ATP detection
  • Preparation of three-dimensional metal-enhanced fluorescence/colorimetric dual-mode paper chip and ATP detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Application of three-dimensional metal-enhanced fluorescence / colorimetric dual-mode paper chip in ATP detection:

[0037] (1) Design the hydrophobic wax printing area and hydrophilic working area of ​​the hollow channel paper chip. The diameter of the hydrophilic area of ​​the right paper chip is 6 mm, and the gray area of ​​the left paper chip is completely corresponding to the hydrophilic area of ​​the right paper chip. The 6 mm hole in the hole is connected by a hydrophilic channel with a length of 5 mm and a width of 2 mm;

[0038] (2) Print the filter paper chip designed in step (1) on a hydrophobic pattern with a Fuji Xerox wax spray printer;

[0039] (3) Heat the paper chip obtained in step (2) at 60-150 ºC for 1-2 minutes to melt the wax and form a hydrophobic layer;

[0040] (4) Put the paper chips obtained in step (3) on the laser cutting machine, cut off the gray area to form holes;

[0041](5) Grow three-dimensional gold-silver nanostars on the working are...

Embodiment 2

[0049] The preparation steps are the same as Example 1, except that the paper chip used is chromatographic paper.

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Abstract

The invention discloses preparation of a three-dimensional metal-enhanced fluorescent / colorimetric dual-mode paper chip and a method for measuring ATP by using a fluorescent / colorimetric dual-mode sensor. A hydrophobic area, a hydrophilic area and a hollow channel are prepared on the paper chip by using a wax printing technology, and the hollow channel is cut through a laser cutting machine. Gold and silver nanostars are grown in a working area 1, then a fluorescent signal material is fixed, and later a fluorescent quenching agent is dropwise added, so that ATP highly-sensitive fluorescent measurement is realized through the fluorescent signal 'off-on'. The prepared paper chip can be folded, and hydrogen peroxide is dropwise added in the reaction area, so as to realize ATP visual colorimetric measurement.

Description

technical field [0001] The invention relates to the field of low-cost, easy-to-carry, and visualized ATP analysis and detection technology, more specifically to the preparation and ATP detection of a three-dimensional metal-enhanced fluorescence / colorimetric dual-mode paper chip. The invention also relates to the use of three-dimensional metal enhanced Fluorescence signal amplification technology. Background technique [0002] All life activities use ATP as the direct energy source. Changes in ATP content in the body (in skeletal muscle and red blood cells) can directly reflect the functional state and exercise capacity of the body. Therefore, the determination of ATP is an important part of the functional evaluation of athletes. ATP is a commonly used measurement indicator. The determination methods include fluorescence method, bioluminescence method, electrophoresis method and high performance liquid chromatography. Some of these methods are complicated to operate; som...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCG01N21/6486G01N21/78
Inventor 梁琳琳葛慎光于京华兰飞飞李丽刘海云任娜
Owner UNIV OF JINAN
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