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An enhanced photodynamic anti-tumor nano-drug loading system and its preparation method and application

A nano-drug-loading, photodynamic technology, applied in the field of anti-tumor drugs, can solve problems such as poor treatment effect, and achieve the effect of enhancing anti-tumor effect, high biological safety, and high anti-tumor activity

Active Publication Date: 2020-09-01
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides an enhanced photodynamic anti-tumor nano drug-carrying system and its preparation method and application in order to overcome the defect that the existing photodynamic therapy method has poor therapeutic effect due to the tumor tissue and the surrounding hypoxic environment.

Method used

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  • An enhanced photodynamic anti-tumor nano-drug loading system and its preparation method and application
  • An enhanced photodynamic anti-tumor nano-drug loading system and its preparation method and application
  • An enhanced photodynamic anti-tumor nano-drug loading system and its preparation method and application

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preparation example Construction

[0036] The present invention also provides the preparation method of the nano-drug loading system described in the above technical solution, comprising the following steps:

[0037] (1) dissolving the nanolipid material and the chemotherapeutic drug in an organic solvent, adding dropwise to a solution containing a near-infrared photosensitizer, and stirring to obtain a nanoliposome coated with the chemotherapeutic drug and the near-infrared photosensitizer;

[0038] (2) modifying a layer of branched gold nanosphere shells on the surface of the nanoliposomes obtained in step (1) by seed growth method to obtain nanoliposomes modified with branched gold nanospheres;

[0039] (3) Mixing the nanoliposomes obtained in step (2) with catalase, incubating, and modifying the surface of the branched gold nanosphere shell with catalase to obtain an enhanced photodynamic anti-tumor nano drug-carrying system.

[0040] In the present invention, the nano-lipid material and the chemotherapeuti...

Embodiment 1

[0053] Dissolve 3 mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station), 30 mg soybean lecithin (purchased from Shenyang Tianfeng Biological Co., Ltd.) and 1 mg paclitaxel (purchased from Shanghai Yuanye Biological Co., Ltd.) in 3 mL of absolute ethanol , sonicated to dissolve it, and dropped into 8 mL of indocyanine green solution with a concentration of 0.5 mg / mL, and magnetically stirred for 1 hour to obtain paclitaxel indocyanine green nanoliposomes. Add 5 μL of 200 mM sodium borohydride solution to 1.0 mL of 0.5 mM gold chloride solution and shake vigorously to prepare gold nanoparticle solution; 400 μL of each gold nanoparticle solution is mixed with paclitaxel indocyanine green nanoliposomes , add 300 μL of 1.5 mM gold chloride solution and 30 μL of 100 mM hydroxylamine hydrochloride, and incubate for 8 hours to obtain branched gold spherical shell-modified paclitaxel indocyanine green nanoliposomes; 75 μg / ml catalase The solution was added to...

Embodiment 2

[0056] Dissolve 4 mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station), 40 mg soybean lecithin (purchased from Shenyang Tianfeng Biological Co., Ltd.) and 3 mg paclitaxel (purchased from Shanghai Yuanye Biological Co., Ltd.) in 5 mL of absolute ethanol , ultrasonicated to dissolve it, and it was added dropwise to 9 mL of indocyanine green solution with a concentration of 0.75 mg / mL, and magnetically stirred for 1.5 hours to obtain paclitaxel indocyanine green nanoliposomes; in 1.5 mL of 0.75 mM Add 7.5 μL of 225 mM sodium borohydride solution to the gold chloride solution, shake vigorously to prepare a gold nanoparticle solution; mix 450 μL of each gold nanoparticle solution with paclitaxel indocyanine green nanoliposomes, add 400 μL of 1.75 mM The gold chloride solution was incubated with 40 μL of 150 mM hydroxylamine hydrochloride for 9 hours to obtain paclitaxel indocyanine green nanoliposomes modified with branched gold spheres; catalase was adde...

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Abstract

The invention provides an enhanced photodynamic anti-tumor nano-drug loading system which relates to the technical field of anti-tumor drugs. The nano-drug loading system is modified with hydrogen oxide enzyme, dendritic gold nanosphere shells and nano-liposomes sequentially from inside to outside, and the nano-liposomes are coated with chemotherapeutic drugs and near-infrared photosensitizers. The nano-drug loading system modified with hydrogen oxide enzyme used for increasing the oxygen content around the tumor tissue and overcoming the limitation of photodynamic treatment effect of the tumor tissue and surrounding hypoxia of the same; on the other hand, the problems about limitations of a single treatment mode and poor anti-tumor effect are solved; the combination of chemotherapy and photodynamic therapy is adopted, mutual promotion under various treatment modes can eliminate tumor cells more efficiently and deeply, and the anti-tumor effect is improved.

Description

technical field [0001] The invention relates to the technical field of anti-tumor drugs, in particular to an enhanced photodynamic anti-tumor nano drug-carrying system and a preparation method and application thereof. Background technique [0002] At present, traditional tumor chemotherapy has problems such as poor targeting, large toxic and side effects, single treatment mechanism, and poor treatment effect. In tumor-specific microenvironments (high hydrogen peroxide, weak acidity, hypoxia, etc.), nano-drug carriers can convert spatiotemporally controllable energy such as light, ultrasound, and magnetic fields into therapeutic factors for tumors, resulting in a variety of biological effects. [0003] Photodynamic therapy is a new technology for disease diagnosis and treatment using photodynamic effect. It is a photosensitization reaction accompanied by biological effects involving oxygen molecules. The process is that laser irradiation of a specific wavelength causes the ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K47/42A61K47/02A61K41/00A61K31/337A61P35/00
CPCA61K9/1271A61K31/337A61K41/0057A61K47/02A61K47/42A61P35/00A61K2300/00
Inventor 高大威何雨初丛聪李磊骆丽垚
Owner YANSHAN UNIV
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