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Preparation of ratiometric electrochemiluminescent paper chip and its application in the detection of lead ions

An electrochemical and paper chip technology, applied in the construction of a three-dimensional paper chip laboratory technology platform, can solve problems such as false positives in test results, and achieve the effect of simple preparation process and low cost

Inactive Publication Date: 2018-10-19
UNIV OF JINAN
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  • Description
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Problems solved by technology

However, in the ordinary electrochemiluminescence detection method, due to the environment of the electrode or the instrument, it is easy to cause false positives in the detection results, and the accuracy of the detection results needs to be further improved.

Method used

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  • Preparation of ratiometric electrochemiluminescent paper chip and its application in the detection of lead ions
  • Preparation of ratiometric electrochemiluminescent paper chip and its application in the detection of lead ions

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Effect test

Embodiment 1

[0028] Preparation of a ratiometric three-dimensional electrochemiluminescence paper chip and its application in Pb 2+ Applications under test:

[0029] (1) Design the hydrophobic wax batch printing pattern of ratiometric electrochemiluminescent three-dimensional paper chips on the computer;

[0030] (2) Cut the sheet of paper into a common A4 size paper, print the hydrophobic wax batch printing pattern designed in step (1) onto the A4 paper, then place the A4 paper with the wax pattern in an oven and heat it at 150 ºC 2 min to melt the wax and soak through the entire thickness of the paper to form a hydrophobic wall;

[0031] (3) Using the screen printing method, print the printing patterns of the working electrode, reference electrode, and counter electrode on the paper obtained in step (2) in sequence, where the right side is the working area with the working electrode printed on it, and the left side is the Reference area, on which the reference electrode and counter ele...

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Abstract

The invention discloses a preparation method of a ratio type electrochemiluminescent paper chip and application of three-dimensional paper-based equipment in lead ion detection. A working area is prepared by a wax printing technology on a paper chip, three electrodes are printed, and then the working area is functionalized. Through multi-layer modification, a phosphate buffer solution containing H2O2 is dropwise added in the working area, and the working area is connected to an electrochemical workstation, so that the highly sensitive and portable detection on Pb<2+> is realized.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence detection, and more specifically relates to the construction of a three-dimensional paper-on-a-chip laboratory technology platform suitable for lead ion detection. Background technique [0002] In recent years, with the rapid economic development, the problem of environmental pollution has become increasingly prominent, and problems such as air pollution, soil pollution, and water pollution have followed one after another. Heavy metal ions such as Pb 2+ , Hg 2+ It is one of the main causes of water pollution, which not only has a great impact on the ecosystem, but also poses a serious threat to human health. For example, long-term exposure to Pb-rich 2+ In the environment, it will damage the human central system, and even damage the human brain, lungs, kidneys and other organs, so the detection of heavy metal ions is of great significance. At present, the detection methods of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 李丽张彦梁琳琳葛慎光刘海云于京华
Owner UNIV OF JINAN
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