Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes

Abstract

A nematode culture device and a simple breeding method for entomopathogenic nematodes, relating to a nematode culture device and a nematode breeding method. The purpose of the method is to solve the problems of long time consumption, low yield and low reproduction efficiency in the live culture method of entomopathogenic nematodes for testing in existing laboratories. The device comprises an inner screen and an outer water storage basin, the edge of the inner screen extends outward to form an outer edge, and the outer edge of the inner screen is placed on the edge of the outer water storage basin. Method: 1. Cut two pieces of circular medium-speed qualitative filter paper with the same diameter, place them in the lid of the petri dish, put the last instar wax moth larvae on the filter paper in the petri dish, and infiltrate the filter paper with the water suspension of nematodes in the infection stage , buckle the bottom of the dish, put it in the incubator, and then put it in the incubator until all the wax moths die; 2. Transfer the dead wax moths to the inner screen of the nematode culture device, and the outer water storage basin Sterile water is housed inside, placed in the incubator, and the nematode suspension in the outer water storage basin is collected. The invention is used for cultivating nematodes.

Description

technical field [0001] The invention relates to a nematode culture device and a nematode breeding method. Background technique [0002] Entomopathogenic nematodes (Entomopathogenic Nematode, EPN) have been successfully used to control underground pests, such as leek maggots, grubs and cutworms. However, because the production cost is much higher than that of chemical agents, manufacturers can only produce according to user orders at present. Because the amount of entomopathogenic nematodes used in laboratory, greenhouse and field experiments is not very large, different types or strains of nematodes will be used in different experiments, and the manufacturer cannot provide them in time, and some nematode products produced by the manufacturer are mixed with different types of nematodes . Therefore, the acquisition of entomopathogenic nematodes for testing in the laboratory is all self-reproduction. [0003] At present, entomopathogenic nematodes used in laboratory bioassay...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of long time consumption, low yield and low reproduction efficiency in the live culture method of entomopathogenic nematodes ...

Images