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Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes

A technology for the cultivation of entomopathogenic nematodes and nematodes, which is applied in animal husbandry and other fields, can solve the problems of low reproduction efficiency, long time consumption, and low yield, and achieve the effect of improving reproduction efficiency, simple method, and increasing yield

Inactive Publication Date: 2016-10-26
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems of long time consumption, low yield and low reproduction efficiency in the live culture method of entomopathogenic nematodes for testing in existing laboratories, and provides a kind of entomopathogenic nematodes for testing in laboratories for laboratory, greenhouse and field experiments. easy breeding method

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  • Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes

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specific Embodiment approach 1

[0019] Specific implementation mode one: combine figure 1 To illustrate this embodiment, the nematode culture device of this embodiment includes an inner screen 1 and an outer water storage basin 2, the edge of the inner screen 1 extends outward to form an outer edge 3, and the edge of the inner screen 1 The outer edge 3 is placed on the edge of the outer water storage basin 2, and the height of the bottom of the inner layer screen 1 from the bottom of the outer water storage basin 2 is 1 cm.

specific Embodiment approach 2

[0020] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the volume of the inner screen 1 is 5-6 decimeters. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0021] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the mesh size of the inner screen 1 is 0.2 cm×0.2 cm. Others are the same as in the first or second embodiment.

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Abstract

A nematode culture device and a simple breeding method for entomopathogenic nematodes, relating to a nematode culture device and a nematode breeding method. The purpose of the method is to solve the problems of long time consumption, low yield and low reproduction efficiency in the live culture method of entomopathogenic nematodes for testing in existing laboratories. The device comprises an inner screen and an outer water storage basin, the edge of the inner screen extends outward to form an outer edge, and the outer edge of the inner screen is placed on the edge of the outer water storage basin. Method: 1. Cut two pieces of circular medium-speed qualitative filter paper with the same diameter, place them in the lid of the petri dish, put the last instar wax moth larvae on the filter paper in the petri dish, and infiltrate the filter paper with the water suspension of nematodes in the infection stage , buckle the bottom of the dish, put it in the incubator, and then put it in the incubator until all the wax moths die; 2. Transfer the dead wax moths to the inner screen of the nematode culture device, and the outer water storage basin Sterile water is housed inside, placed in the incubator, and the nematode suspension in the outer water storage basin is collected. The invention is used for cultivating nematodes.

Description

technical field [0001] The invention relates to a nematode culture device and a nematode breeding method. Background technique [0002] Entomopathogenic nematodes (Entomopathogenic Nematode, EPN) have been successfully used to control underground pests, such as leek maggots, grubs and cutworms. However, because the production cost is much higher than that of chemical agents, manufacturers can only produce according to user orders at present. Because the amount of entomopathogenic nematodes used in laboratory, greenhouse and field experiments is not very large, different types or strains of nematodes will be used in different experiments, and the manufacturer cannot provide them in time, and some nematode products produced by the manufacturer are mixed with different types of nematodes . Therefore, the acquisition of entomopathogenic nematodes for testing in the laboratory is all self-reproduction. [0003] At present, entomopathogenic nematodes used in laboratory bioassay...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李春杰王从丽
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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