Method for extracting flavones in Sorbus alnifolia through coupled supersonic wave-aqueous two-phase system

A two-phase aqueous system, the technology of Sorbus flavonoids, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of long time, low extraction efficiency, and large damage to heat-sensitive components. Achieve the effect of simple production method, easy to master, easy to recycle

Inactive Publication Date: 2016-10-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic method has mild conditions and high extraction rate, but the time is long and the extraction efficiency is not high
The microwave method is widely used in the extraction o

Method used

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  • Method for extracting flavones in Sorbus alnifolia through coupled supersonic wave-aqueous two-phase system

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Embodiment 1

[0027] A method for coupling extraction of Sorbus elm flavonoids by ultrasonic wave and two-phase system, the specific steps are as follows:

[0028] Step 1) extraction and separation of flavonoids from the leaves of Sorbus chinensis, using propanol-ammonium sulfate two-phase aqueous phase to obtain upper phase liquid and lower phase liquid;

[0029] Step 2) cooling the lower phase liquid in step 1), crystallizing, filtering, and recovering ammonium sulfate crystals;

[0030] Step 3) Purify, concentrate and dry the upper phase liquid in step 1) with absolute ethanol.

Embodiment 2

[0032] A method for coupling extraction of Sorbus elm flavonoids by ultrasonic wave and two-phase system, the specific steps are as follows:

[0033] Step 1) Extraction and separation of flavonoids from Sorbus chinensis leaves, and use propanol-ammonium sulfate two-phase aqueous phase to obtain the upper and lower phase liquids: dry the fresh Sorbus chinensis leaves and crush them; first use ammonium sulfate 4.50- 6.00g, propanol 6mL-7mL, water 10mL to form a stable two-phase system, add 0.5-0.7g of Sorbus elm leaves to the two-phase system, and perform ultrasonic extraction at 300W and 50°C for 20- 40min, filter with suction, remove Sorbus elm residue, let the filtrate stand for 1-3h, separate into upper phase liquid and lower phase liquid, separate upper phase liquid and lower phase liquid while hot, and obtain upper phase liquid and lower phase liquid .

[0034] Step 2) cooling the lower phase liquid in step 1), crystallizing, filtering, and recovering ammonium sulfate cry...

Embodiment 3

[0039] A method for coupling extraction of Sorbus elm flavonoids by ultrasonic wave and two-phase system, the specific steps are as follows:

[0040] Step 1) Extraction and separation of flavonoids from Sorbus chinensis leaves, and use propanol-ammonium sulfate two-phase aqueous phase to obtain the upper and lower phase liquids: dry the fresh Sorbus chinensis leaves and crush them; first use 5.25g of ammonium sulfate , propanol 6mL, and water 10mL to form a stable two-phase system. Add 0.5 g of Sorbus elm leaves to the two-phase system, perform ultrasonic extraction for 40 minutes at a power of 300W and a temperature of 50°C, and filter to remove the Sorbus slag, the filtrate was left standing for 2 hours, stratified into an upper phase liquid and a lower phase liquid, and the upper phase liquid and the lower phase liquid were separated while hot to obtain the upper phase liquid and the lower phase liquid. After cooling the upper phase liquid and the lower phase liquid to room...

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Abstract

The invention relates to a method for extracting flavones in Sorbus alnifolia through coupled supersonic wave-aqueous two-phase system. The method is characterized by comprising the following concrete steps: step 1) carrying out extraction and separation of flavones in Sorbus alnifolia leaves, wherein a propanol- ammonium sulfate aqueous two-phase system is used and upper-phase liquid and lower-phase liquid are obtained; step 2) subjecting the lower-phase liquid obtained in the step 1) to cooling, crystallization, filtering and recovery of ammonium sulfate crystals; and step 3) subjecting the upper-phase liquid obtained in the step 1) to purification with absolute ethyl alcohol, concentration and drying so as to obtain a finished product. The method has the advantages that phase-splitting is rapid; extraction and separation processes are integrated; the concentration and purity of the flavones in an upper phase are increased; material consumption is reduced; toxicity is low; ethanol and ammonium sulfate are easy to recycle; the recovery rate of flavones in Sorbus alnifolia leaves is high; the method is simple, easy to control, free of influence by environment, low in cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of separation and purification of effective components of natural products, and relates to a method for extracting the flavonoids of Sorbus elm by coupling ultrasonic wave and two-phase system. Background technique [0002] Sorbus elm ( Sorbus alnifolia ) is Rosaceae (Rosaceae) Sorbus genus ( Sorbus ) plants, distributed in Heilongjiang, Jilin, Liaoning, Henan, Shaanxi, etc., and are distributed on the north and south slopes of Qinling Mountains in Shaanxi. Tianshan Sorbus elm contains rich flavonoids, anthocyanins, bisphenols, cyanogenic glycosides and other components, and has strong anti-oxidation, anti-radiation, anti-cancer, cough and asthma effects in pharmacological effects. However, there is no report on the active ingredients of Sorbus elm. [0003] Aqueous two-phase extraction separation technology ( Aqueous Two Phase Extraction, ATPE ) is a new separation technology, which is widely used in the separa...

Claims

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Application Information

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IPC IPC(8): A61K36/73
Inventor 吉文丽赵选李秀信陈劲松于航
Owner NORTHWEST A & F UNIV
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