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A novel peptide ligand for modifying quantum dots

A technology of polypeptide ligands and quantum dots, applied in the direction of peptides, can solve the problems of affecting the tertiary structure of the target protein activity, difficult to improve the binding force of the polypeptide sequence, slow binding speed, etc., to solve the problems of insufficient stability, fast binding speed, The effect of good binding affinity

Active Publication Date: 2019-05-28
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: in order to overcome the problems of slow binding speed and poor stability of the existing polypeptide ligands and QDs, and limit its popular use in the biological field, in order to solve the problem of more than 6 histidines due to steric hindrance The polypeptide sequence of residues is difficult to improve the binding force, and although the histidine tag is one of the most popular fusion tags, it may affect the activity of the target protein and their tertiary structure technical problems, the present invention provides a A novel peptide ligand with fast binding speed and good stability to QDs

Method used

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  • A novel peptide ligand for modifying quantum dots
  • A novel peptide ligand for modifying quantum dots
  • A novel peptide ligand for modifying quantum dots

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Embodiment 1

[0019] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected to expose the amino group, and forms a peptide bond with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby linking the amino acid to the On the resin, the peptide chain is extended from the C-terminus to the N-terminus.

[0020] 1. Basic materials:

[0021] (1) Resin and linking molecule: the resin selected by the solid-phase Fmoc method is Rink resin. This kind of resin has very good swelling property, which can make the condensation reaction between the peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to immobilize the polypeptide molecules on the resin.

[0022] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.

[0023] 2. Re...

Embodiment 2

[0044] Refer to Example 1 for the steps of polypeptide synthesis. According to DDG(NH) 6 The order of the sequence is synthesized from right to left, and the synthetic novel peptide ligand sequence is as follows:

[0045] ATTO-DDG(NH) 6

Embodiment 3

[0047] Refer to Example 1 for the steps of polypeptide synthesis. According to EES(NH) 6 The order of the sequence is synthesized from right to left, and the synthetic novel peptide ligand sequence is as follows:

[0048] ATTO-EES(NH) 6

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Abstract

The invention discloses a novel peptide ligand (ATTO-NH) modifying quantum dots, and belongs to the technical field of nano-biology. The novel peptide ligand comprises dye ATTO590 (1) used for FRET with the quantum dots, sequences (2) which improve polypeptide water solubility and carries negative charges and asparagine and histidine spacer sequences (3) used for combining the quantum dots, and all the sequences are combined through peptide bonds. The ligand is simple in synthetic method, the C terminal of the ligand is combined with the quantum dots through six groups of asparagine and histidine spacer sequences, the combination speed of the ligand and the quantum dots are greatly increased, the stability is greatly improved, and the novel peptide ligand can serve as a nanometer fluorescence probe to be widely applied to biological markers.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a novel polypeptide ligand for modifying quantum dots. Background technique [0002] Metal cations cause serious pollution to the environment due to their toxicity, so it is necessary to develop a new and sensitive method for detecting metal ions. Molecular sensors based on specific metal ions have become a research hotspot in recent years, and the development of sensors based on quantum dots (QDs) is highly dependent on the self-assembly of novel ligands with them. Affinity metal-driven quantum dots have been effectively used in functionalized quantum dots because of their high controllability. Studies have shown that the number of histidines (N≤6) has an impact on the self-assembly of peptide ligands and quantum dots, and found that long histidine sequences can enhance binding affinity. However, due to steric hindrance, it is difficult for peptide sequences with more than 6 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083
CPCC07K5/0806C07K5/081
Inventor 王建浩张晨澄邱琳蒋鹏举柳丽王车礼滕一万陈瑶
Owner CHANGZHOU UNIV