An integrated equipment for gel staining and decolorization and its control method

Abstract

The invention discloses gel dyeing-decoloration integrated equipment. The gel dyeing-decoloration integrated equipment comprises a gel dyeing-decoloration integrated machine and a control unit, wherein the gel dyeing-decoloration integrated machine comprises a shaking table driver, a shaking table, a dyeing-decoloration cylinder, an electromagnetic valve, a camera and a reagent cylinder; the control unit comprises a micro-control single chip microcomputer, is electrically connected with the shaking table driver, the electromagnetic valve, a liquid-level sensor, the camera, a warning device and a control panel and is used for receiving input signals and implementing control; and a remote monitoring unit is further set as monitoring equipment, is installed with application software and can be remotely connected with the gel dyeing-decoloration integrated equipment to implement remote monitoring and control. According to the gel dyeing-decoloration integrated equipment, the manual operations in the dyeing-decoloration processes can be reduced, the dyeing-decoloration control is relatively precise, and the success rate is substantially increased; and meanwhile, the contact between a human body with harmful gas and liquid is reduced, so that the risk of accidental injury of an operator is reduced. The gel dyeing-decoloration integrated equipment is novel in design, convenient to use and high in intelligent level and can be remotely and automatically operated.

Description

technical field [0001] The invention relates to the field of gel dyeing and decoloring equipment, in particular to the field of gel dyeing and decoloring integrated equipment. Background technique [0002] Polyacrylamide gel electrophoresis, abbreviated as PAGE, is a commonly used electrophoresis technique using polyacrylamide gel as a supporting medium for the separation and detection of proteins and oligonucleotides. During the preparation of polyacrylamide gels, Coomassie Brilliant Blue R250 or G250 is often used for staining and then decolorized appropriately. At present, the staining and decolorization of polyacrylamide gels are generally carried out manually. Since the dyeing and decolorization process requires long-term uninterrupted replacement of the dyeing solution or decolorization solution, manual operation often takes considerable energy, and the time arrangement is relatively limited. Changing the staining solution or decolorizing solution, or failing to stop...

AI Technical Summary

Problems solved by technology

Since the dyeing and decolorization process requires long-term uninterrupted replacement of the dyeing solution or decolorization solution, manual operation often takes considerable energy, and the time arrangement is relatively limited. Changing the staining solution or decolorization solution, or failing to stop the staining or decolorization operation in time will easily lead to the failure of gel production and the failure of the entire experiment

On the other hand, in the manual operation process, it is inevitable to come into contact with various toxic or carcinogenic reagents such as staining or decolorizing solutions, which is extremely harmful to the physical and mental health of operators.

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