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Dehydrogenase electrode and application thereof

A technology of dehydrogenase and glutamate dehydrogenase, which is applied in the field of electrochemical sensors, can solve the problems of lowering the detection limit and cannot meet the detection of glutamate, and achieves the effects of lowering the detection limit, simple preparation method and high sensitivity

Active Publication Date: 2016-10-26
CAPITAL NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the published literature, the detection limit of glutamic acid detected by electrochemical method is 50 μM, but the actual glutamic acid content in the mouse brain is only a few μM, that is, the existing electrochemical method cannot meet the requirements of the actual mouse brain. The detection of glutamate in the brain, so improving the sensitivity of electrochemical methods to detect glutamate and reducing the detection limit is an urgent problem to be solved

Method used

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  • Dehydrogenase electrode and application thereof
  • Dehydrogenase electrode and application thereof
  • Dehydrogenase electrode and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Activation: place the glassy carbon electrode in a 0.1M, pH 7.4 phosphate buffer solution and activate it for 20 minutes by cyclic voltammetry at an activation potential of -1.6V to +2.7V to prepare the activated glassy carbon electrode;

[0033] 2) Adsorption: placing the activated glassy carbon electrode prepared in step 1) in a 1 mM methylene green solution for 2 hours to obtain a glassy carbon electrode adsorbed with a methylene green catalyst;

[0034] 3) Enzyme immobilization: The glassy carbon electrode adsorbing methylene green prepared in step 2) was drip-coated with glutamate dehydrogenase, ascorbate oxidase and cross-linking agent, cross-linked at room temperature and then fixed in a refrigerator at 4°C. A dehydrogenase electrode (or an electrochemical sensor) is prepared.

Embodiment 2

[0036] The difference between this example and Example 1 is that the methylene green solution is replaced by the methylene blue solution.

Embodiment 3

[0038] The difference between this example and Example 1 is that the glassy carbon electrode adsorbed methylene green prepared in step 2) was drip-coated with glutamate dehydrogenase, alanine aminotransferase, ascorbate oxidase and a cross-linking agent.

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Abstract

The invention relates to the field of electrochemical sensors, and particularly relates to a dehydrogenase electrode and application thereof. The dehydrogenase electrode comprises a substrate electrode, a catalyst layer coating the substrate electrode, and an enzyme layer coating the catalyst layer, and the enzyme layer comprises glutamate dehydrogenase and ascorbic acid oxidase. The characteristic that ascorbic acid oxidase can oxidize ascorbic acid, interference influence of ascorbic acid in actual animal brain is successfully eliminated, and online realtime detection of target objects is realized. By further adjusting working environment of the dehydrogenase electrode, sensitivity is improved, detection limit is lowered, and using the dehydrogenase electrode in detection of low-concentration glutamic acid in vivo is made possible. A preparation method of the dehydrogenase electrode is simple and easy and high in repeatability, and the dehydrogenase electrode is low in detection limit and high in sensitivity and can be used for online realtime detection of glutamic acid in the actual animal brain.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to a dehydrogenase electrode. Background technique [0002] Glutamic acid is an important neurotransmitter in the brain nervous system, and together with other neurotransmitters (such as dopamine, acetylcholine, neuropeptides, etc.) and neuromodulators (such as ascorbic acid), glucose, lactic acid, ions and other substances together form a complex The chemical system of the brain together realizes the functional operation of neurons. Studies have reported that glutamic acid has an important relationship with schizophrenia, Parkinson's syndrome, stroke and other brain neurological diseases. It carries out its own transport and signal transduction through a large number of "carriers" to complete the physiological and pathological state of the body Therefore, it is very necessary to quantitatively monitor the content of glutamate in the body, especially when dynamic changes occu...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 林雨青李博李琳赵旭
Owner CAPITAL NORMAL UNIVERSITY
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