Determination method of ε-polylysine content and molecular weight in beverages
A technology of polylysine and detection method, which is applied in the field of detection of ε-polylysine content and molecular weight in beverages, can solve the problems of unreported ε-polylysine content and its molecular weight, and achieves a high degree of automation , good reproducibility and high sensitivity
Active Publication Date: 2018-06-08
FUJIAN INSPECTION & RES INST FOR PROD QUALITY
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[0003] However, how to efficiently and accurately detect the content and molecular weight of the added ε-polylysine from food has not been reported.
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[0017] The present invention is further described below.
[0018] The method for detecting the content and molecular weight of ε-polylysine in a drink according to the present invention, the specific steps are as follows:
[0019] a. Extraction of ε-polylysine in drinks: Take 10 mL of the sample to be tested, heat the sample to be tested at 70°C for 10 min, centrifuge at 10,000 r / min for 10 min, and then collect the supernatant;
[0020] b. Add 1 g of D152 macroporous weakly acidic cation exchange resin to the collected supernatant, then mix the mixture of drink supernatant and D152 macroporous weakly acidic cation exchange resin at a speed of 200 r / min for 5 min, so that ε-polylysine is fully adsorbed on the D152 macroporous weakly acidic cation exchange resin;
[0021] c. Use 10 mL of 1 mol / L hydrochloric acid to elute ε-polylysine from the D152 macroporous weakly acidic cation exchange resin, and the eluate is used as the object of high performance liquid chromatography; ...
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Abstract
The invention relates to a method for detecting content and molecular weight of epsilon-polylysine in beverage. According to characteristics of epsilon-polylysine polycations, adsorption extraction is performed on the beverage by using macroporous weakly acidic cations D152, and then elution is performed by using hydrochloric acid of 1 mol / L, so that the epsilon-polylysine of 95% in the beverage can be extracted and obtained, and interference caused by other matters in the beverage to subsequent detection is avoided; by detection of the ultraviolet absorption strength of the extracted epsilon-polylysine under 215 nm, the content of the epsilon-polylysine in a sample is calculated and obtained; by detection of the retention time of the epsilon-polylysine in a gel filtration chromatographic column, the molecular weight of the epsilon-polylysine in the sample is calculated and obtained. By adoption of the method for detecting the content and the molecular weight of the epsilon-polylysine in the beverage, the epsilon-polylysine contained in the beverage can be highly efficiently extracted, and the content and the molecular weight of the epsilon-polylysine in the beverage can be detected at the same time.
Description
technical field [0001] The invention relates to a method for detecting the content and molecular weight of ε-polylysine in drinks. Background technique [0002] ε-polylysine was first discovered as a secondary metabolite of Streptomyces in 1977 when Shima et al. screened alkaloids. It is composed of multiple l-lysine alpha - A linear polymer in which carboxyl groups and ε-amino groups are iteratively connected through amide bonds, with a common molecular weight of 3.2-4.5 kDa and a degree of polymerization of 25-35. ε-polylysine has a wide pH tolerance range and good thermal stability. At pH 3.0, ε-polylysine still has biological activity after being treated at 100°C for 30 minutes or 120°C for 20 minutes. Experiments have proved that ε-polylysine has broad-spectrum antibacterial properties, and has strong inhibitory effects on both Gram-negative and positive bacteria, and also has inhibitory effects on yeast, fungi and even viruses. And the inhibitory effect is closely r...
Claims
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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘友华徐虹陈永煊冯小海李莎许召贤余清
Owner FUJIAN INSPECTION & RES INST FOR PROD QUALITY