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Preparation method and application of electrochemical aptamer sensor for the detection of streptomycin

An aptamer sensor and sensitive streptomycin technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of limiting the large-scale practical application of detection methods, limiting the scope of application, complex pretreatment, etc., to increase the fixed amount and stability. , the effect of improving sensitivity and specificity, and reducing manufacturing costs

Active Publication Date: 2016-11-02
UNIV OF JINAN
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Problems solved by technology

The LC-MS method can accurately detect streptomycin and other aminoglycoside antibiotics, but the sample needs to be pre-treated more complicated and expensive, which greatly limits the detection method. Large-scale practical application
Immunoassay is based on the specific combination of antigen-antibody, which is very suitable for the detection of low-concentration target substances with high specificity and high sensitivity. Due to the cross-reaction of complex compounds in some actual samples such as honey, it will affect the immune system. Chromatographic reaction results, which limits the scope of application of the method in real samples

Method used

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  • Preparation method and application of electrochemical aptamer sensor for the detection of streptomycin
  • Preparation method and application of electrochemical aptamer sensor for the detection of streptomycin

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Embodiment 1

[0035] (1) Preparation of porous carbon nanospheres PCNS, first synthesize carbon nanospheres, mix 8.0g cellulose with 2.4g FeSO 4 -(NH 4 ) 2 SO 4 ·6H 2 O was dissolved in 60 mL of deionized water, and then the solution was placed in an autoclave, reacted at 180 °C for 24 h, washed by centrifugation, and dried in vacuum at 60 °C for later use; the dried sample was dissolved in 40 mL of MgCl 2 (0.25M) solution, reacted at 120°C for 12h, washed and dried, and activated the dried sample at 800°C for 2h under the condition of nitrogen, and the activated sample was treated with hydrochloric acid (0.2M) and Washing with deionized water, drying and grinding to obtain porous carbon nanospheres.

[0036] (2) Preparation of polyaniline-copper oxide-gold cage (PANI-CuO-GM): 0.2g CuSO 4 ·5H 2 O and 0.1g of cetyltrimethylammonium bromide (CTAB) were dissolved in 80mL of deionized water, then 0.12g of ammonium persulfate was added, stirred by magnetic force, and 2.5mL of C 6 h 5 NH ...

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Abstract

The invention relates to a preparation method of an electrochemical aptamer sensor for the detection of streptomycin. The method is as below: modifying an electrode surface layer by layer by a porous carbon nanospheres (PCNS), polyaniline-copper oxide-gold cage (PANI-CuO-GM) composite, standing to a dry state at normal temperature, then fixing to a streptomycin aptamer to the electrode surface modified by the above nano material, and blocking non-specific binding sites by using bovine serum albumin as a blocking agent; conducting electrochemical test on the prepared streptomycin aptamer sensor, and detecting the streptomycin concentration through current change before and after the combination of the streptomycin with aptamer. The preparation and detection method of the streptomycin aptamer sensor system well solve the tedious procedures of the current sensor preparation, and uses carbon material as a signal amplification material to greatly reduce the manufacturing cost. The electrodes of the same batch of sensors have good identity, and can achieve mass production and commercialization.

Description

technical field [0001] The invention relates to the field of streptomycin trace analysis and detection, in particular to the preparation and application of an electrochemical aptamer sensor based on carbon materials. Background technique [0002] Streptomycin is an aminoglycoside antibiotic produced by Streptomyces griseus, and it is the second antibiotic produced and used clinically after penicillin. Because streptomycin has stable properties, wide antibacterial spectrum, simple production process, and good curative effect, it is especially effective against knots. [0003] It has a unique inhibitory effect on Gram-negative bacteria, so it has been widely used, such as an antibacterial drug in the treatment of human diseases, a veterinary drug in animal feeding, a pesticide in crop maintenance and In beekeeping, it is used as an antibacterial drug for the treatment of bee bacterial diseases. [0004] However, the use of streptomycin can also cause some serious side effect...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 阴军玲郭文娟裴梅山
Owner UNIV OF JINAN
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