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Construction method for inducible specific liver injury mouse model

A technology of liver injury and construction method, applied in the field of biomedicine, can solve the problems of high mortality, high surgical risk, difficulty in reproduction of uPA mice, etc.

Inactive Publication Date: 2016-11-23
SHANGHAI PUBLIC HEALTH CLINICAL CENT +1
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Problems solved by technology

Existing animal models, such as the Alb-uPA mouse model, have been used in the study of liver cell transplantation. However, due to the difficulty in breeding uPA mice and the high mortality rate, the transplantation operation needs to be completed within 2 weeks of the birth of the mouse and the surgical risk is high. A series of problems such as large size limit its wide application

Method used

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  • Construction method for inducible specific liver injury mouse model
  • Construction method for inducible specific liver injury mouse model
  • Construction method for inducible specific liver injury mouse model

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1. Breeding and identification of Alb-cre / DTR double transgenic mice

[0032] After crossing Alb-cre with DTR mice, Alb-cre / DTR double transgenic mice were obtained, and the hybrid mice were genotyped by PCR to confirm that they contained Alb-cre and DTR genes;

[0033] 2. Intraperitoneal injection of diphtheria toxin

[0034] Take SPF grade Alb-cre / DTR mice, dissolve diphtheria toxin at a dose of 0.3~1.25ng / g of mouse body weight in 200µl of PBS solution, and create a model by intraperitoneal injection to obtain an inducible specific liver injury model ; C57BL / 6 mice of the same age as controls;

[0035] After intraperitoneal injection of diphtheria toxin, the blood of the mice was collected to separate the serum, and the ALT and AST values ​​were detected to determine whether liver damage occurred;

[0036] 3. Detect changes in biochemical indicators related to liver damage to evaluate whether the liver damage model is successful.

[0037] The results show that the...

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Abstract

The invention belongs to the field of biomedicine, and relates to a construction method for an inducible specific liver injury mouse model. The construction method comprises the steps that 1, an Alb-cre / DTR double-transgenic mouse is bred and identified; 2, diphtheria toxins are transferred into the mouse; 3, changes of biochemical indexes related to liver injuries are detected so as to evaluate whether liver injury modelling is successful or not. The specific liver injury mouse model constructed through the method provides a feasible animal model support for liver transplantation research, has an important theoretical significance and application value on research on deep discussion of liver cell transplantation and liver regeneration and the like and also can serve as a human-derived research platform for researching other liver diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a specific liver damage model, in particular to a method for constructing an inducible specific liver damage model. Background technique [0002] According to statistics, liver disease is one of the most threatening diseases to human beings. In addition to viral hepatitis, liver cirrhosis and liver cancer are also very serious. At present, the annual direct medical expenses for hepatitis and liver disease in China are as high as More than 100 billion yuan. In clinical treatment, liver transplantation is one of the very important means to solve liver system diseases and liver metabolic disorders, but liver transplantation still faces many unresolved problems, and more relevant research is needed to improve the liver cells after transplantation. The level of regeneration and proliferation; liver cell transplantation is more feasible and less expensive than liver tissue transplantation. ...

Claims

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Application Information

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IPC IPC(8): A61K38/16
Inventor 周晓辉任晓楠陈丽香沈月雷杨华彭秀华秦波音
Owner SHANGHAI PUBLIC HEALTH CLINICAL CENT
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