Method for establishing symbiotic relationship between ectotrophic mycorrhiza fungi and pinus plants

An ectomycorrhizal and symbiotic relationship technology, applied in botany equipment and methods, plant cultivation, horticulture, etc., can solve the problems of difficult development and utilization, artificial cultivation, etc., to achieve enhanced disease resistance and dark green leaves , the effect of strong growth

Active Publication Date: 2016-12-07
YUNNAN UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The two wild fungi, Ganba and matsutake, are typical ectomycorrhizal fungi, which cannot be cultivated artificially at present, and are difficult to develop and utilize. The growth and development of their fruiting bodies are closely related to the symbiotic plants of the genus Pinus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The content and parameters of step (1) to step (6) are the same as those in Example 1 of D. spp., the genus Pinus is Yunnan pine, and the infection success rate of finally forming ectomycorrhizae can reach 85.8%.

Embodiment 2

[0066] The content and parameters of step (1) to step (6) are the same as those in Example 2 of D. yunnanensis. The genus Pinus is Yunnan pine, and the infection success rate of finally forming ectomycorrhiza can reach 92.1%.

Embodiment 3

[0068] The content and parameters of step (1) to step (6) are the same as those in Example 3 of D. yunnanensis. The genus Pinus is Yunnan pine, and the infection success rate of finally forming ectomycorrhizae can reach 85.1%.

[0069] Matsutake Infestation Example 4

[0070] The content and parameters of step (1) to step (6) are the same as those in Example 1 of D. spp., and the pinus plant is alpine pine, and the infection success rate of finally forming ectomycorrhizae can reach 87.5%.

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Abstract

The invention relates to a method for forming mycorrhizae after two types of ectotrophic mycorrhiza fungi, namely sparassis crispa and tricholoma matsutake infect seedlings of several kinds of pinus plants and belongs to the fields of artificial ectotrophic mycorrhiza fungus infection seedling-growing application technology and agricultural cultivation technology. The method comprises the steps of 1, acquisition and cultivation of sterile seeds of the pinus plants; 2, selection and treatment of a culture substrate; 3, selection and treatment of seedling-growing hole trays; 4, preparation before infection treatment; 5, infection treatment of the sparassis crispa and the tricholoma matsutake; 6, seedling stage management after infection treatment. The seedlings with the mycorrhizae successfully formed through infection grow stronger than the seedlings not subjected to infection, the leaf color is thick green, euphylla grows out in advance, disease resistance is enhanced, the symbiotic relationship between the sparassis crispa, the tricholoma matsutake and the several kinds of pinus plants under controllable conditions is creatively and successfully created, a study case can be provided for the symbiotic relationship between plants and fungi in the study of the ectotrophic mycorrhiza fungi, and a solid scientific foundation is laid for realizing artificial cultivation of the ectotrophic mycorrhiza fungi.

Description

technical field [0001] The invention relates to a method for forming mycorrhizae by ectomycorrhizal fungi such as D. spp. and Matsutake on several kinds of pinus plant seedlings, and belongs to the field of artificial ectomycorrhizal fungus infection seedling application technology and agricultural cultivation technology. Background technique [0002] Dried bacteria ( Thelephoraganbajun Zang) and matsutake (also known as matsutake mushroom) [ Tricholomamatsutake (lto et lmai) Singer] are two well-known delicious wild mushrooms, which have high nutritional value and medicinal value, and are deeply loved by people. With the improvement of people's living standards, the supply in the market is currently in short supply, and the price is expensive and dry. The most expensive fresh weight of bacteria can reach 1800 yuan per 1 Kg. Dried bacterium has strong aroma, tender and sweet, tough, dry, mellow and long aftertaste, endless chewing taste, and has a rich aroma similar to co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G9/10
CPCA01G9/0299A01G18/00
Inventor 张光飞沙涛张亚平
Owner YUNNAN UNIV
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