Image iterative reconstruction method orienting large-field-of-view high-resolution microscopic imaging

Abstract

The invention discloses an image iterative reconstruction method orienting large-field-of-view high-resolution microscopic imaging. Firstly an LED array acts as a lighting source of a microscope, each LED element is lighted up in turn to irradiate a sample and then corresponding images are acquired; the low-resolution images photographed in a way of irradiating the sample by using the LED element arranged at the center of the LED array are utilized to initialize the amplitude and the phase of the high-resolution images; synthetic aperture operation is performed on each acquired image in turn in a frequency domain by using an incremental gradient method; the incremental gradient iteration coefficient is updated with a cost function value acting as the criterion; and iteration is stopped when the incremental gradient iteration coefficient is less than a given threshold. Complex parameter adjustment is not required, and the method has high resistance to the noise in the acquired images so that the large-field-of-view high-resolution images can be quite stably and accurately reconstructed.

Description

technical field [0001] The invention belongs to optical microscopic imaging technology, in particular to an image iterative reconstruction method for large field of view and high resolution microscopic imaging. Background technique [0002] In the field of microscopic imaging, higher resolution has always been the goal of pursuit, but there is a key problem while improving the resolution, that is, the spatial bandwidth product of the microscope does not increase with the resolution. From the perspective of the imaging system, in order to achieve high resolution, the numerical aperture of the microscope objective lens must be increased, but the improvement of spatial resolution and the expansion of the field of view are often a pair of contradictions that are difficult to reconcile. In short, the whole picture of the inspected object can be seen under the low magnification lens, but only a small part of the inspected object can be seen when it is replaced with a high magnific...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an image iterative reconstruction method for large field of view and high resolution microscopic imaging, so as to solve the contradiction between the resolution of the microscopic system and the size of the field of view, and improve the spatial bandwidth product of the microscopic system

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