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A kind of industrialized production method of Escherichia coli producing L-alanine

A technology of Escherichia coli and production method, which is applied in the field of L-alanine production by Escherichia coli, can solve problems such as shortage and inability to implement industrialized production, achieve the effects of good product quality, reduce production cost, and solve potential safety hazards

Active Publication Date: 2020-04-03
BINZHOU JINLANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although it is theoretically feasible to use microbial fermentation technology instead of enzyme-catalyzed technology to produce L-alanine and has many technical advantages, there are still a lot of problems in the transformation from theory to industrial production, such as the adaptability of strains, The selection of large-scale production equipment, the parameter adjustment of large-scale production, the separation and purification of large-scale products, etc., the existence of these problems makes industrial production impossible. At present, there is still a lack of a feasible microbial fermentation technology to produce L-alanine. industrial production method

Method used

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  • A kind of industrialized production method of Escherichia coli producing L-alanine
  • A kind of industrialized production method of Escherichia coli producing L-alanine
  • A kind of industrialized production method of Escherichia coli producing L-alanine

Examples

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Comparison scheme
Effect test

Embodiment 1

[0054] The industrial production method for producing L-alanine by Escherichia coli, 151203 batches of steps are specifically:

[0055] (1) Use the slope 20151111R112H021 batch of Escherichia coli CGMCC No.10628 to inoculate into the fermentation serial number 305-15012 batches for fermentation and culture, and prepare the fermentation broth. The volume of the prepared fermentation broth is 24.5T, and the unit of the fermentation broth is 152g / L , the prime minister of fermented liquid produces 3724kg.

[0056] (2) At the end of fermentation, raise the temperature of the fermented liquid to 65°C and keep the temperature for 30 minutes; at the end, add 74 tons of purified water 3 times the volume for dilution, and the total volume of the feed liquid is 98 tons, and the temperature drops to 35°C. Turn on the 1#-4# disc centrifuge to separate the bacteria; the centrifugal speed of the centrifuge is 60Hz5800 rpm, and the feeding speed is 1.2m 3 / h. The 6 tons of bacterial concen...

Embodiment 2

[0069] The industrial production method for producing L-alanine by Escherichia coli, 160108 batches of steps are specifically:

[0070] (1) Use the slope 20151211R135K007 batch of Escherichia coli CGMCC No.10628 to inoculate into the batch of fermentation serial number 304-16008 for fermentation and culture, and prepare the fermentation liquid. The volume of the prepared fermentation liquid is 23.9T, and the unit of the fermentation liquid is 158g / L , the prime minister of fermented liquid produces 3776kg.

[0071] (2) At the end of fermentation, raise the temperature of the fermentation broth to 67°C and keep the temperature for 30 minutes; at the end, add 71.7 tons of purified water 3 times the volume for dilution, and the total volume of the feed liquid is 95.9 tons, and the temperature drops to 36°C. Turn on the 1#-4# disc centrifuge to separate the bacteria; the centrifugal speed of the centrifuge is 60Hz5880 rpm, and the feeding speed is 1.2m 3 / h. The 5.6 tons of bact...

Embodiment 3

[0084] The industrial production method for producing L-alanine by Escherichia coli, 160110 batches of steps are specifically:

[0085] (1) The slope 20160112A100C006 batch of Escherichia coli CGMCC No.10628 was used to inoculate into the fermentation serial number 303-16011 batch for fermentation and culture, and the fermentation liquid was prepared. The volume of the prepared fermentation liquid was 25.2T, and the fermentation liquid unit was 151g / L , the prime minister of fermented liquid produces 3805kg.

[0086] (2) At the end of fermentation, raise the temperature of the fermented liquid to 61°C and keep the temperature for 30 minutes; at the end, add 75.6 tons of purified water 3 times the volume for dilution, and the total volume of the feed liquid is 100.8 tons, and the temperature drops to 37°C. Turn on the 2#-5# disc centrifuge to separate the bacteria; the centrifugal speed of the centrifuge is 60Hz5780 rpm, and the feeding speed is 1.2m 3 / h. The 6.5 tons of bacte...

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Abstract

The invention discloses an industrial production method for producing L-alanine through escherichia coli. The process from fermentation liquor processing to packaging completion is carried out in the hermetic environment for production, it is ensured that cross contamination or personnel contact contamination does not occur on the product and no black point foreign matter is produced on the product, and the product quality is good. Through two times of decoloration treatment, and the product purity is high and reaches 99.9% or above through third-party detection (Shanghai Qinlu detection). The yield of L-alanine reaches 82% or above, the wastewater production amount is small, environmental protection treatment pressure is small, and the production cost is reduced by 15% or above.

Description

technical field [0001] The invention relates to the technical field of L-alanine production, in particular to an industrial production method for E. coli to produce L-alanine. Background technique [0002] L-alanine is widely used in the fields of food and medicine. In the field of food, the addition of L-alanine can significantly improve the utilization rate of protein in food and beverages, and it can quickly recover from fatigue and boost the spirit after eating. L-alanine can improve the pickling effect and flavor of pickled products, improve the quality of alcoholic beverages, etc. L-alanine has a unique flavor-improving effect, and it can enhance the flavor of food and beverages when combined with other amino acids. It can also improve the sour taste of organic acids, and make organic acids closer to the sour taste of natural fruit acids after adding. In the field of medicine, L-alanine is an important raw material for the synthesis of vitamin B6, and it is also the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C07C227/40C07C229/08C12R1/19
CPCC07C227/40C12P13/06C07C229/08
Inventor 王京光魏哲王长海李晓芸
Owner BINZHOU JINLANG BIOTECH
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