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Quality Control Method of Cimicifuga Gegen Decoction Composition

A quality control method, the technology of Cimicifuga Root Decoction, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of few indicators, many medicinal flavors, and single quality control, and achieve objective conclusions, convenient operation, and control of preparation quality. Effect

Active Publication Date: 2019-03-01
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome that the traditional Chinese medicine compound preparation in the prior art basically only has one or two index components in content control, the quality control is single, the index is few, and it is difficult to fully reflect that there are many flavors and many components. , the defects of the quality status of the composition of Cimicifuga Gegen Decoction with many targets, thereby providing a quality control method for the composition of Cimicifuga Gegen Decoction

Method used

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  • Quality Control Method of Cimicifuga Gegen Decoction Composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment 1. Preparation of Radix Cimicifugae Radix Decoction Composition

[0070] The first Cimicifuga Gegen Decoction composition: Weigh the four-flavored decoction pieces with the following weight ratio: 300g Cimicifuga; 9g (2g Cimicifuga, 3g Pueraria Radix, 2g Radix Paeoniae Alba, 2g Zhilicorice) are placed in a 2L decoction casserole, add 300ml water, soak for 30 minutes, cover and cook, heat (voltage value about 220V) to boiling (about 10 Minutes), keep boiling slightly (voltage value about 170V) to about 100ml of medicinal liquid (about 38 minutes), use two layers of medical gauze to filter out medicinal residues, and obtain.

[0071] The second Cimicifuga Gegen Decoction composition: Weigh the following decoction pieces Cimicifuga 666.7g, Pueraria 1000g, Radix Paeoniae Alba 666.7g, Radix Glycyrrhizae 666.7g by weight, cut into coarse particles with a particle size of 2mm-8mm, add 12 times the amount of water Water, decocted for 30 minutes, filtered to obtain...

Embodiment 2

[0072] Example 2. Content determination of isoferulic acid, puerarin, paeoniflorin, liquiritin, and glycyrrhizic acid in the composition of Cimicifuga Gegen Decoction Certainly

[0073] (1) Determination of chromatographic conditions

[0074] The chromatographic column adopts octadecylsilane bonded silica gel chromatographic column; column temperature: 305°C; the detection instrument adopts DAD detector, adopts wavelength switching technology, the specific switching procedure is as follows, the mobile phase is methanol-0.1% phosphoric acid solution as the mobile phase Gradient elution; the number of theoretical plates is not less than 4000 based on the puerarin peak; the specific procedure is as follows:

[0075] Gradient conditions for the determination of multi-index component content

[0076]

[0077] Multi-index detection wavelength table

[0078]

[0079] (2) Preparation of mixed reference solution

[0080] Take appropriate amount of isoferulic acid, paeonifl...

Embodiment 3

[0092] Example 3. Research on Fingerprint of Cimicifuga Gegen Decoction Composition

[0093] (1) Chromatographic conditions The chromatographic column adopts octadecylsilane bonded silica gel chromatographic column; flow rate 1.0ml / min; column temperature: 30°C; detection instrument adopts ultraviolet detector, detection wavelength 220nm; theoretical plate number is expressed as puerarin peak The meter is not less than 4000; the reference solution is the methanol solution of daidzin; the mobile phase is acetonitrile-0.1% phosphoric acid solution as the system, and the gradient elution is adopted, and the gradient elution sequence is:

[0094]

[0095] (2) Preparation of reference solution

[0096] Take daidzin reference substance, add methanol to prepare a 200μg / ml reference substance solution, and obtain it.

[0097] (3) Preparation of the test solution

[0098] Take 5ml of the first Cimicifugae Radix Decoction composition, centrifuge at 12000r / min for 10min, take the ...

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Abstract

The invention discloses a quality control method for a rhizoma-cimicifugae and kudzu-vine-root soup composition, and belongs to the field of traditional Chinese medicine analysis. According to the quality control method, a fingerprint spectrum is established with the efficient liquid chromatography, wherein the chromatographic conditions are characterized in that a chromatographic column is an octadecyl silane bonded silica gel chromatographic column, the flow speed is 0.9 ml / min to 1.1 ml / min, the column temperature is 25 DEG C to 35 DEG C, a detection instrument is an ultraviolet detector, the detection wavelength is 210 nm to 250 nm, the theoretical plate number is higher than or equal to 4,000 according to puerarin peaks, a reference solution is a daidzin methanol solution, and a flowing phase system is acetonitrile-0.1%-phosphoric acid solution and is eluted in the following order (please see the specification). The fingerprint spectrum comprehensively reflects the quality information of rhizoma-cimicifugae and kudzu-vine-root soup, and therefore the aim of more comprehensively and efficiently controlling the quality of rhizoma-cimicifugae and kudzu-vine-root soup preparation products can be achieved.

Description

technical field [0001] The invention belongs to the field of analysis of traditional Chinese medicines, and in particular relates to a quality control method for the composition of Cimicifugae Radix and Root Decoction. Background technique [0002] Traditional Chinese medicine decoction is the mainstream of clinical medicine, but because of decoction and inconvenient to carry, the quality of the decoction prepared by decoction often varies greatly depending on the person and the decoction utensils. The use of modern science and technology to develop traditional Chinese medicine preparations that are easy to carry and easy to take is not only the inheritance of traditional Chinese medicine, but also a better promotion of classic famous prescriptions. Clinical application. The preparation process of granules is relatively simple, easy to shape, easy to take and carry, and the form of taking is most consistent with traditional decoctions, and can maintain the characteristics o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈世彬覃旭刘志刚陈周全谭沛马鹏岗姚少姿刘志东
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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