Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of miR-1255 in preparation of drugs for treating carcinoma of urinary bladder

A mir-1255, coding gene technology, applied in gene therapy, drug combination, antineoplastic drugs, etc., can solve the problem of lack of effective drugs for the treatment of metastatic bladder cancer

Inactive Publication Date: 2016-12-21
BEIJING BELIFE BIO MEDICAL TECH LTD
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, surgery or radiotherapy is effective for localized bladder cancer, and there is still a lack of effective drugs for the treatment of metastatic bladder cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of miR-1255 in preparation of drugs for treating carcinoma of urinary bladder
  • Application of miR-1255 in preparation of drugs for treating carcinoma of urinary bladder
  • Application of miR-1255 in preparation of drugs for treating carcinoma of urinary bladder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Acquisition of metastatic bladder cancer cell lines stably and highly expressing miR-1255

[0032] 1. Acquisition of bladder cancer cell lines

[0033] For the colorectal cancer cell line SW780 (ATCC), the culture medium is DMEM medium (Invitrogen, 31053036) containing 10% fetal bovine serum (Hyclone) and 0.2% penicillin and streptomycin (Invitrogen).

[0034] A. Cell recovery

[0035] (1) Take out the freezing tube of SW780 (ATCC), immediately put it in a water bath at 37°C-40°C, and shake it quickly until it melts completely.

[0036] (2) Transfer the cell suspension into 10ml cold PBS, put it into a 15ml centrifuge tube, centrifuge the cells with a horizontal centrifuge (1000 rpm, 8 minutes) and discard the supernatant.

[0037] (3) Suspend the cells with an appropriate amount of fresh medium, transfer the cell suspension into a culture bottle, and culture in a carbon dioxide incubator at 37°C.

[0038] B. Cell culture

[0039] Put the bladder cancer c...

Embodiment 2

[0065] Example 2. Functional study of stable and high expression of miR-1255 in SW780-high cell line

[0066] 1. Cell growth curve

[0067] (1) Take the SW780-high obtained in Example 1 in a good growth state 24 hours after transfection, and digest it with a general passage method to make a cell suspension. Counting, accurately planting cells in a 96-well plate, the total number of cells per well is required to be consistent (2000 / well), the amount of culture medium added should also be consistent, and a blank control (that is, the same volume of medium) is set, each well The final volume is 100 μL. Set at 37°C, 5% CO 2 Incubate for 24 hours in the incubator.

[0068] (2) Add 10 ul of CCK-8 solution (purchased from Japan Dojin Chemical Research Institute, article number: CK04) to each well. The amount of CCK-8 solution can be increased or decreased according to specific conditions.

[0069] (3) Normal culture conditions (37°C, 5% CO 2 incubator) for 4 hours (the incubati...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of any of substances (represented in (1) to (4)) in preparation of products capable of promoting the migration, diffusion and / or invasion of tumor cells. The substances (represented in (1) to (4)) are as follows: (1) miR-1255; (2) a recombinant vector of a coding gene containing the miR-1255; (3) a recombinant virus of a coding gene containing the miR-1255; (4) a recombinant virus vector of a coding gene containing the miR-1255. According to the application, proven by experiments, the miR-1255 cannot affect the growth of cells SW780 after overexpression, however, the invading and migrating capability of cells of carcinoma of urinary bladder can be remarkably enhanced, and thus, the miR-1255 possibly plays an important role in a metastasis process of the carcinoma of urinary bladder, so that the miR-1255 is related to the metastasis of the carcinoma of urinary bladder.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a microRNA in the preparation of a drug for treating bladder cancer. Background technique [0002] microRNA (miR) is a single-stranded small molecule RNA with a size of about 21-23 bases. It guides the silencing complex (RISC) to degrade mRNA or inhibit the translation of mRNA by base pairing with target gene mRNA, usually after transcription Horizontal regulation of protein expression (recently found that microRNA can also regulate gene expression at the transcriptional level). With the deepening of microRNA research, more and more evidences show that microRNA molecules play the role of proto-oncogene or tumor suppressor gene in all aspects of tumorigenesis and development. [0003] Bladder cancer refers to malignant tumors that occur on the bladder mucosa. It is the most common malignant tumor of the urinary system and one of the ten most common tumors in the w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K31/337A61P35/00A61P35/04
CPCA61K31/337A61K48/0008A61K48/005
Inventor 崔健姜薇
Owner BEIJING BELIFE BIO MEDICAL TECH LTD