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A liquid shallow circulation culture device and high-density rapid culture method for acetic acid bacteria

A high-density technology of acetic acid bacteria, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of low yield of acetic acid bacteria, insufficient use of nutrients, etc., and achieve short-term high-efficiency and energy-saving Source high-density liquid culture, improve utilization rate, and ensure the effect of normal dissolved oxygen

Active Publication Date: 2018-07-10
陕西天酵集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the problems of insufficient use of nutrients and low output of acetic acid bacteria in traditional acetic acid bacteria culture, and provide a liquid shallow circulation of acetic acid bacteria that has a simple structure and can quickly and automatically cultivate acetic acid bacteria in large quantities. Cultivation device, and a method for quickly cultivating acetic acid bacteria with high density using the device

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  • A liquid shallow circulation culture device and high-density rapid culture method for acetic acid bacteria
  • A liquid shallow circulation culture device and high-density rapid culture method for acetic acid bacteria
  • A liquid shallow circulation culture device and high-density rapid culture method for acetic acid bacteria

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Embodiment 1

[0029] Such as figure 1 As shown, the liquid shallow circulation culture device for acetic acid bacteria of the present embodiment consists of a liquid outlet pipe 1, a rectangular tank 2, a growth medium storage tank 3, a one-way flow pump 4, a constant flow pump 5, a heating tank 6, a two-way flow It is composed of a pump 7, a bacteria suspension recovery tank 8, and a supplementary culture medium storage tank 9.

[0030] The growth medium storage tank 3 communicates with the top of the culture tank through a pipeline through a one-way feeding pump 4. The culture tank is formed by stacking four rectangular tanks 2 with openings on the upper surface. The length of the rectangular tank 2 is 100 cm. , The width is 15cm, the height is 4cm, and the material is acrylic board. The bottom of each rectangular groove 2 is processed with a liquid outlet pipe 1, and the liquid outlet pipes 1 on adjacent rectangular grooves 2 are arranged in a staggered manner. The diameter of the outl...

Embodiment 2

[0041] In the present embodiment, the cultivation tank is formed by stacking 8 rectangular tanks 2 with openings on the upper surface. The length of the rectangular tank 2 is 60 cm, the width is 25 cm, and the height is 1 cm. The diameter of the outlet pipe 1 is 1.0 cm and the height is 2mm. All the other components and the connections of the components are the same as in Embodiment 1.

[0042] Adopt the method of high-density rapid culture acetic acid bacteria of the device of the present embodiment, in step 1, the acclimation medium used is to add 3g glucose, 1g yeast extract, 0.5g soybean protein powder, 0.1g alanine to every 100mL distilled water Acid, 0.005g sodium acetate, 0.001g coenzyme Q10, 0.001g folic acid, after sterilization, add 2mL absolute ethanol and 1mL glacial acetic acid. Repeat the acclimatization culture for 5 more times, and the glacial acetic acid added to every 100 mL of distilled water in the acclimatization medium was increased by 1.5 mL successivel...

Embodiment 3

[0044] In the present embodiment, the culture tank is formed by stacking 12 rectangular tanks 2 with openings on the upper surface. The length of the rectangular tank 2 is 160 cm, the width is 5 cm, and the height is 3 cm. The diameter of the outlet pipe 1 is 1.0 cm and the height is 3mm. All the other components and the connections of the components are the same as in Embodiment 1.

[0045] Adopt the method for high-density rapid culture of acetic acid bacteria in the device of the present embodiment, in step 1, the acclimation medium used is to add 6g glucose, 2g yeast extract, 2g soybean protein powder, 0.5g alanine, Prepare 0.01g sodium acetate, 0.01g coenzyme Q10, 0.001g folic acid, add 2mL absolute ethanol and 1mL glacial acetic acid after sterilization. Repeat the acclimatization culture twice more, and the glacial acetic acid added to every 100mL of distilled water in the acclimatization medium was increased by 3mL successively; in step 3, the shallow growth medium us...

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Abstract

The invention discloses an acetic bacteria liquid-state shallow-layer circulating culture device and a high-density fast culture method. The device is composed of a rectangular tank, a liquid outgoing pipe, a growth culture medium storage tank, a one-way fed-batch pump, a constant flow pump, a heating tank, a two-way fed-batch pump, a bacterium suspension recycling tank and a supplement culture medium storage tank. The device is simple in structure and low in cost and energy consumption; by adopting the device to culture acetic bacteria, culture area is increased greatly, using rate of culture substance is increased, normal dissolved oxygen and thallus growing planar space needed in the growing process of the acetic bacteria are guaranteed, concentration of liquid culture acetic bacteria is increased, and short-time, high-efficiency, resource-saving, high-density, automatic and circulating liquid-state culture of acetic bacteria is realized finally. Compared with liquid-state shaker oscillation culture and liquid-state fermentation tank stirring culture methods in existing factory acetic bacteria production, the method is simple and easy to implement and can be realized easily in existing factories, and large-amount, quick and automatic culture of acetic bacteria can be realized.

Description

technical field [0001] The invention relates to a device and method for high-density rapid culture of acetic acid bacteria with liquid shallow circulation. Background technique [0002] Acetic acid bacteria (Acetic acid bacteria) are Gram-negative strict aerobic bacteria that mainly live in raw materials containing sugar, acid or alcohol. Under the microscope, the cells of acetic acid bacteria are mostly short rods, single, and most of them are in pairs. No spores, the size is 0.6~1.0μm×1.7~1.9μm. [0003] As one of the most commonly used microorganisms in the food industry, acetic acid bacteria not only play a key role in the brewing process of grain vinegar, but also are used in the brewing of fruit vinegar, the preparation of polysaccharide films, the development of homopolycellulose products, etc. multiple uses. The traditional culture process of acetic acid bacteria is divided into solid state culture and liquid state culture, among which liquid state culture is divid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N1/20C12R1/01
CPCC12M23/42C12M29/18C12N1/20
Inventor 张宝善颜统晶韦露莎沈洋洋
Owner 陕西天酵集团股份有限公司