Time-resolved fluorescence analysis system and detection device

Abstract

The invention discloses a time-resolved fluorescence analysis system and a detection device. The system comprises a control unit as well as a guide rail, a first motor, an optical detection unit, a light source and an upper computer unit which are electrically connected with the control unit, wherein the guide rail is used for accommodating reagent strips and is driven by the first motor to convey the reagent strips to the optical detection unit for scanning detection; the control unit controls the light source to be switched on to irradiate the reagent strips; after a first preset period of time, the light source is switched off and the optical detection unit is switched on, fluorescence emitted by the reagent strips is detected and converted into electric signals, and the electric signals are output to the control unit for processing; after a second preset period of time, the optical detection unit is switched off, and the first motor is controlled to rotate for a step length; the steps are repeated until the scanning detection of the reagent strips is finished; the upper computer unit is used for receiving the scanned data, data in the start time period is extracted from the scanned data, and a fluorescence decay curve in a test time period is calculated according to the data in the start time period. According to the time-resolved fluorescence analysis system and the detection device, the integrity and the precision of light intensity signal acquisition are improved.

Description

technical field [0001] The invention relates to the technical field of fluorescence-labeled sample detection, in particular to a time-resolved fluorescence analysis system and a detection device using the time-resolved fluorescence analysis system. Background technique [0002] Time-resolved fluorescence immunochromatography is a non-isotope immunoassay technique. It uses lanthanides to label antigens or antibodies. According to the luminescent characteristics of lanthanide chelates, time-resolved techniques are used to measure fluorescence, and both wavelength and time are detected simultaneously. It can effectively eliminate the interference of non-specific fluorescence and greatly improve the analysis sensitivity, so it has been widely used. [0003] Existing time-resolved fluorescent immunochromatographic analyzers usually use rare earth metals, such as europium, in order to obtain a larger Stokes (Stokes shift, that is, the difference between the same electronic transit...

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a time-resolved fluorescence analysis system, aiming at solving the problem of inability to obtain a complete set of fluorescent microspheres when the amount of detected fluorescent microspheres is small in the detection process of the prior art using a time-resolved fluorescence immunochromatography analyzer. Fluorescence detection curve, resulting in poor accuracy of detection results

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