Extraction method of neomycin sulfate

Abstract

The invention relates to an extraction method of neomycin sulfate, comprising the steps of pre-treating neomycin fermentation broth, filtering the fermentation broth by micro-filtration membrane, concentrating the broth by nanofiltration membrane, crystallizing the broth in acetone solvent, obtaining crude neomycin by suction filtration, dissolving the crude neomycin in the water, salting the sulfuric acid, dewatering by activated carbon, and then obtaining neomycin sulfate product by fine filtering, spraying and drying. The extraction rate can be improved above 6% in such a manner that the crystallization technology is used for purifying and concentrating to extract the neomycin sulfate, and it is beneficial for improving the economic benefit. The method reduces wastewater discharge and is beneficial for environment protection. The method has small energy consumption without releasing and emitting pungent smell, and is beneficial for the occupational health of staffs on the position.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, in particular to a method for extracting neomycin sulfate. Background technique [0002] Neomycin is the first discovered 2-DOS (2-deoxystreptomyces amine) aminoglycoside antibiotic, which is a mixture isolated from the metabolites of Streptomyces fradiae by Waksman et al in 1949 , Contains A, B, C three components, mainly B, C. Chemical name: 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-α-D-glucopyranosyl)-5-O-[3-O-(2,6- Diamino-2,6-dideoxy-β-L-idopyranosyl)-β-D-ribofuranosyl]-D-streptamethamine sulfate. The product is very soluble in water, almost insoluble in ethanol, ether, acetone or chloroform. [0003] Neomycin sulfate has a strong bactericidal effect on Gram-negative bacteria and some Gram-positive bacteria. It has inhibitory effect on actinomycetes, leptospira and amoeba. Strong antibacterial effect in alkaline solution. Clinically, it is mainly used for the treatment of inte...

AI Technical Summary

Problems solved by technology

In this process, the fermented liquid is diluted with a large amount of water, and directly enters the 732-type resin column. The mycelium in the fermented liquid, unmetabolized culture medium nutrients and other macromolecular impurities are easy to stick to the resin column, affecting the yield and affecting the resin. During the working process of the column, cleaning will produce a large amount of waste water; the yield of resin decolorization is low, and resin regeneration will generate a large amount of waste water; the process of film concentration and ammonia removal is easy to cause damage to materials and consume more energy under high temperature conditions.

In addition, adding NaHSO 3 Acidification and film concentration to remove ammonia are easy to release and emit pungent odors, causing air pollution and detrimental to the occupational health of workers

[0005] At present, there are reports about improving the yield of neomycin sulfate and reducing the amount of waste water, and there are reports on the method of improving the fermentation process and replacing membrane concentration with nanofiltration, but the crystallization and extraction process of neomycin base in acetone has not been mentioned yet.