Synbiotic used for feeds, preparation method thereof, and application of synbiotic in livestock breeding animal feeds

A synbiotic and feeding technology, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems that there is no research report on the joint application of lactose and lactic acid bacteria, and the application of lactose has not yet appeared, so as to promote Proliferation of intestinal probiotics, ensuring the healthy development of the intestinal tract, and improving the body's immunity

Active Publication Date: 2017-02-08
XINGJIA BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the application of epilactose in animal feed has not yet appeared, and there is no research report on the joint application of epilactose and lactic acid bacteria.

Method used

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  • Synbiotic used for feeds, preparation method thereof, and application of synbiotic in livestock breeding animal feeds
  • Synbiotic used for feeds, preparation method thereof, and application of synbiotic in livestock breeding animal feeds
  • Synbiotic used for feeds, preparation method thereof, and application of synbiotic in livestock breeding animal feeds

Examples

Experimental program
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Effect test

Embodiment 1

[0050] A feed synbiotic of the present invention is a microcapsule structure, and the core material of the microcapsule structure is mainly composed of 0.2Kg epilactose and 1Kg containing bacterium number of 1 × 10 8 CFU / g of lactic acid bacteria powder, the packaging material covering the core material is mainly composed of 0.2Kg sodium alginate, 1.2Kg whey protein powder and 1.2Kg soybean protein powder.

[0051] The preparation method of the feed synbiotic of the present embodiment may further comprise the steps:

[0052] (1) Prepare the crude enzyme solution of epigalactase:

[0053] (a) Select commercially available E.coli JM109 (DE3) as the bacterial strain;

[0054] (b) pET21a (Novagen, USA) was selected as the expression vector;

[0055] (c) Prepare medium:

[0056] LB medium: Dissolve 10g tryptone, 5g yeast powder and 10g NaCl in 1000mL deionized water, adjust the pH to 7.0 with 10M NaOH, and sterilize with moist heat for 30min; add 2% agar powder to the solid LB m...

Embodiment 2

[0068] A feed synbiotic of the present invention is a microcapsule structure, and the core material of the microcapsule structure is mainly composed of 0.4Kg epilactose and 1.6Kg containing bacteria number of 1 × 10 8 CFU / g of lactic acid bacteria powder, the packaging material covering the core material is mainly composed of 0.4Kg sodium alginate, 3.2Kg whey protein powder and 2.8Kg soybean protein powder.

[0069] The preparation method of the feed synbiotic of the present embodiment may further comprise the steps:

[0070] (1) Preparation of epigalactase:

[0071] Wherein, the mode of preparing culture medium and epigalactase recombinant expression in Escherichia coli is the same as that of Example 1, and then resuspends the thalline collected after the induced expression with binding buffer (50mMNaCl, 20mM Tris-HCl, 5mM imidazole), and ultrasonic instrument After crushing the bacterial cells, centrifuge at 15,000rpm at 4°C for 20min, take the supernatant and load it onto ...

Embodiment 3

[0080] A feed synbiotic of the present invention is a microcapsule structure, and the core material of the microcapsule structure is mainly composed of 0.4Kg epilactose (Bailingwei Technology Co., Ltd.) and 1.6Kg containing bacteria number is 1 × 10 8 CFU / g of lactic acid bacteria powder, the packaging material covering the core material is mainly composed of 0.4Kg sodium alginate, 3.2Kg whey protein powder and 2.8Kg soybean protein powder.

[0081] The preparation method of the feed synbiotic of the present embodiment:

[0082](1) Add 0.4Kg sodium alginate, 3.2Kg whey protein powder and 2.8Kg soybean protein powder into 100Kg deionized water, and stir until a uniform packaging material solution is formed;

[0083] (2) The number of bacteria contained in 0.4Kg and 1.6Kg of epilactose produced by Bailingwei Technology Co., Ltd. is 1×10 8 CFU / g of lactic acid bacteria powder is dissolved in the packaging material mixed solution, and stirred evenly; then the obtained uniform mix...

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Abstract

The invention discloses a symbiotic used for feeds. The synbiotic is a microcapsule structure, the core material of the microcapsule structure is mainly prepared from epilactose and lactic acid bacteria, and the packing material of the microcapsule structure is mainly prepared from sodium alginate, lactalbumin and soybean proteins. A preparation method of the synbiotic used for feeds comprises the following steps: adding epilactose and the lactic acid bacteria to a packing material solution, and fully and uniformly mixing the epilactose, the lactic acid bacteria and the packing material solution; and carrying out compressed air extrusion to form liquid drops, dropwise adding the liquid drops to a coagulating agent solution, coagulating the mixture to obtain microcapsules, and drying the microcapsules to obtain the synbiotic used for feeds. The synbiotic used for feeds can be added to the feeds in order to promote growth of animals, increase the body weight, promote propagation of intestinal probiotics, guarantee healthy growth of the intestinal tract and improve the body immunity, is safe, has no side effects, and can be used for a long term.

Description

technical field [0001] The invention belongs to the field of feed additives, and in particular relates to a synbiotic for feed, a preparation method thereof and an application thereof in animal feed for livestock and poultry breeding. Background technique [0002] The long-term addition of feed antibiotic growth-promoting agents in feed has caused the drug residues in animal-derived food to exceed the standard seriously, posing great harm to the national health and ecological environment of our country, and has become a constraint to the healthy and sustainable development of my country's feed industry. Bottleneck problem. Therefore, it is of great practical significance and long-term development prospect to develop and develop safe, efficient and green alternatives to feed antibiotic growth promoters. [0003] The concept of synbiotics (Synbiots) was proposed by Gibson and Roberfroid in 1995. It is a mixture of probiotics and prebiotics, which has the ability to increase th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23K10/18A23K20/22A23K20/147A23K10/14A23K40/30A23K50/30A23K50/10A23K50/75
Inventor 黄逸强彭红星张亚伟石波邓敏姚亚军
Owner XINGJIA BIO ENG CO LTD
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