Human-body lubricant containing fermented supernatant of lactic acid bacteria
A technology of human body lubricant and fermentation supernatant, applied in the field of sanitary products, can solve the problems of poor lubricity, side effects on the human body, easy to breed bacteria, etc., and achieve the effects of good lubricating effect, good bacteriostatic effect and important application value.
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Embodiment 1
[0042] Embodiment 1, the isolation and identification of lactic acid bacteria
[0043] 1. Isolation of bacteria
[0044] 1. Collection of samples
[0045] The vaginal secretions of women (all volunteers who gave informed consent to the test content) were collected as samples, a total of 10 samples.
[0046] 2. Isolation of bacteria
[0047] (1) Take a sample and dilute it to 10 times the volume with sterile water to obtain a sample dilution. Take 1mL sample diluent, stir and inoculate in solid medium at 30-40°C, and incubate at 37°C for 24-72h.
[0048] (2) After completing step (1), select a single colony, spot-inoculate it on a double-layer solid plate containing 1.6% (mass percentage) bromocresol violet, and culture it at 37°C for 24-72h.
[0049] (3) After completing step (2), select a single colony producing yellow pigment, streak and inoculate it on a double-layer solid plate containing 1.6% (mass percentage) bromocresol violet, and culture at 37°C.
[0050] (4) Aft...
Embodiment 2
[0057] Embodiment 2, the preparation of human body lubricant
[0058] 1. Preparation of fermentation supernatant
[0059] 1. Take bacterial strain N1 and inoculate it into MRS medium (the initial bacterial concentration of lactic acid bacteria is 6×10 5 cfu / mL), shake at 30°C (shaking speed: 220rpm, radius of rotation: 50mm) and culture for 24h, collect the entire culture system and name it system 1.
[0060] 2. After completing step 1, take system 1, inoculate it into MRS medium at a volume ratio of 1:9, and culture at 30°C for 24 hours with shaking (shaking speed: 220rpm, rotation radius: 50mm), collect the entire culture system, and name it system 2 .
[0061] 3. After completing step 2, take system 2, centrifuge at 4000rpm for 10min, and collect the supernatant. The supernatant was sterilized by filtration (filter pore size: 0.45 mm), and the fermentation supernatant of strain N1 was obtained.
[0062] According to the above method, replace strain N1 with strain N2, st...
Embodiment 3
[0067] Antibacterial effect and lubricating effect of the human body lubricant prepared by embodiment 3, embodiment 2
[0068] One, the antibacterial effect of the human body lubricant prepared in embodiment 2
[0069] According to the method of the antibacterial ring test of "Disinfection Technical Specification" 2002 edition 2.1.8.2, measure the bacteriostatic effect of the human body lubricant to be tested to be tested bacterium. The human body lubricants to be tested are human body lubricant A1, body lubricant A2, body lubricant A3, body lubricant B1, body lubricant B2, body lubricant B3, body lubricant C1, body lubricant C2, and body lubricant C3 , Body lubricant D1, Body lubricant D2, Body lubricant D3, Body lubricant E1, Body lubricant E2, Body lubricant E3, Body lubricant F1, Body lubricant F2, Body lubricant F3, Body lubricant G1 , Personal Lubricant G2, Personal Lubricant G3 or Personal Lubricant K. The tested bacteria were Staphylococcus aureus or Candida albicans...
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Abstract
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