Method for determination of fat-soluble vitamins by using semi-automatic sample treatment liquid chromatographic technology

Abstract

The invention discloses a method for simultaneous determination of various fat-soluble vitamins in serum by using a semi-automatic sample treatment liquid chromatographic technology; the HPLC method is adopted, and the chromatographic conditions comprise that a mobile phase A is a 0.05% trifluoroacetic acid aqueous solution, a mobile phase B is a 0.02% trifluoroacetic acid methanol-ethanol mixed solution, and gradient elution is adopted; the various fat-soluble vitamins are selected from vitamin A, vitamin D2, vitamin D3, alpha-tocopherol, beta-tocopherol, gamma-tocopherol, delta-tocopherol, K1, K2 or Q10. Through optimization of a sample pretreatment method and high performance liquid chromatographic conditions, the method for simultaneous determination of the various fat-soluble vitamins in the serum is established, can carry out precise qualitative and quantitative analysis, is a detection method having the advantages of simple and fast sample processing, high flux, reliable result and low cost, has the advantages of strong specificity, high sensitivity, simple operation, low cost and the like, has easily analyzed and objective results, and is especially suitable for clinical popularization and application.

Description

Technical field [0001] The invention relates to a method for simultaneously determining multiple fat-soluble vitamins in serum by using semi-automatic sample processing liquid chromatography technology, and belongs to the technical field of vitamin detection. Background technique [0002] Vitamins are a class of low-molecular compounds necessary for the body to maintain normal metabolism and function. It is one of the seven major nutritional elements of the human body. It is divided into fat-soluble and water-soluble vitamins. Vitamins in the human body must be kept in a suitable range to protect the body. Beneficial, no matter lack or increase, it will bring harm to human body. Vitamin A, D2, D3, α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, K1, K2, and Q 10 They are all fat-soluble vitamins. When the human body lacks these vitamins, different symptoms will appear: A deficiency can easily cause night blindness and eye inflammation, and D deficiency can easily cause ric...

AI Technical Summary

Problems solved by technology

With the continuous impact of the international vitamin market on the Chinese and even Asian markets, as well as the scientific research achievements and technological breakthroughs made by domestic scientific research institutions and enterprises in the vitamin industry, the awareness of health care among Chinese citizens has been continuously enhanced. Various types of vitamins, however, due to the lack of scientific guidance, the status quo of the use of vitamin products is very chaotic. In order to improve various functions of the body, some people spend a lot of money on health care products and supplement vitamins blindly. According to the survey of the Chinese Nutrition Society in 2014, Diseases caused by overnutrition or unbalanced nutrition in Chinese residents are increasing year by year

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