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Method and device for measuring clotting time, reagent and kit for measuring clotting time

A coagulation time and measurement method technology, applied in measuring devices, biological testing, instruments, etc., can solve the problem of heparin activity reduction

Active Publication Date: 2019-08-02
SYSMEX CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In U.S. Patent No. 5,705,396, it is described that the activity of heparin decreases when an APTT assay reagent containing a copper salt or a zinc salt is used in the APTT assay

Method used

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  • Method and device for measuring clotting time, reagent and kit for measuring clotting time
  • Method and device for measuring clotting time, reagent and kit for measuring clotting time
  • Method and device for measuring clotting time, reagent and kit for measuring clotting time

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Experimental program
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Embodiment approach

[0055] [1. Measurement method of coagulation time]

[0056] The method for measuring coagulation time according to the present embodiment (hereinafter, also simply referred to as "measurement method") is characterized by including (A) a step of mixing a blood test sample, an activator, a phospholipid, and a nickel ion-forming compound to obtain a sample, and (B) a step of preparing a measurement sample by mixing the sample obtained in the step (A) and a calcium salt, and measuring the coagulation time of the measurement sample. In the measurement method according to the present embodiment, in the step (A), when the sample is obtained, the blood test sample, the activator, the phospholipid, and the nickel ion-forming compound are mixed. Therefore, according to the measurement method according to the present embodiment, even when a test sample containing heparin is used, the coagulation time can be measured with high sensitivity.

[0057] Furthermore, in this specification, "sa...

Embodiment approach 1

[0065] (Embodiment 1) The step of mixing the blood test sample, the activator and the phospholipid, and then adding nickel ions to form a compound

Embodiment approach 2

[0066] (Embodiment 2) A step of adding an activator and a phospholipid after mixing the blood test sample and the nickel ion-forming compound

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Abstract

Disclosed is a method for measuring a clotting time, including the steps of: (A) mixing a blood sample, an activator, a phospholipid, and a nickel ion-forming compound to obtain a specimen; and (B) mixing the specimen obtained in step (A) with a calcium salt to prepare a measurement specimen and measuring the clotting time of the measurement specimen.

Description

[0001] 【Technical field】 [0002] The present invention relates to a method for measuring coagulation time, a device thereof, a reagent for measuring coagulation time, and a kit. [0003] 【Background technique】 [0004] Activated partial thromboplastin time (APTT) is used in monitoring the concentration of heparin as an anticoagulant agent (see US Pat. No. 5,705,396). In US Pat. No. 5,705,396, it is described that the activity of heparin is reduced when a reagent for APTT measurement containing a copper salt or a zinc salt is used in the APTT measurement. [0005] However, even when a test sample containing heparin is used, it is desired to measure the clotting time with high sensitivity. [0006] 【Summary of Invention】 [0007] The scope of the invention is to be determined only by the appended claims and is not to be affected in any way by this summary of the invention. [0008] The present invention provides the following technical solutions. [0009] (1) the assay metho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86
CPCG01N33/86G01N2400/40
Inventor 熊野穰山口温辉铃木健史家子正裕
Owner SYSMEX CORP