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A fresh Cordyceps sinensis extract with anti-melanoma activity

A technology of fresh Cordyceps sinensis and anti-melanin, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of poor growth inhibition of B16 melanoma cells, achieve strong inhibition, and less toxic side effects , the effect of broad application prospects

Active Publication Date: 2019-11-12
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the document "Inhibitory effects of ethylacetate extract of Cordyceps sinensis mycelium on various cancer cellsin culture and B16melanoma in C57BL-6mice" reported that the ethyl acetate extract of Cordyceps sinensis artificially fermented mycelium has a positive effect on MCF-7 breast cancer cells and B16 melanoma cells. Very good inhibitory effect, while its hot water extract has no inhibitory effect
The literature "Mycelium cultivation, chemical composition and antitumour activity of a Tolypocladium sp. fungus isolated from wild Cordyceps sinensis" also reported that Cordyceps sinensis fermented mycelium extract had poor growth inhibitory effect on B16 melanoma cells in vitro

Method used

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  • A fresh Cordyceps sinensis extract with anti-melanoma activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take 10g of fresh Cordyceps sinensis, cut it into pieces, add 10 times the amount of water, homogenate at high speed for 7 minutes, the homogenization speed is 15000rpm, control the temperature at 20±2°C, crush the fresh Cordyceps sinensis into fine particle suspension, ultrasonically assisted extraction for 30 minutes, control The temperature is 20±2°C, and then the fresh Cordyceps extract is placed in a -20°C refrigerator to freeze for 50 minutes, taken out, homogenized and dispersed evenly, and then ultrasonically extracted for 30 minutes, the temperature is controlled at 20±2°C, centrifuged for 12 minutes, and the speed is 9000rpm, collect the supernatant, and freeze-dry to obtain fresh Cordyceps sinensis extract.

Embodiment 2

[0040] Take 10g of fresh Cordyceps sinensis, cut it into pieces, add 8 times the amount of water, homogenate at high speed for 6 minutes, the homogenization speed is 14000rpm, control the temperature at 15±2°C, crush the fresh Cordyceps sinensis into fine particle suspension, ultrasonically assist extraction for 20 minutes, control The temperature is 15±2°C, and then the fresh Cordyceps sinensis extract is placed in a -20°C refrigerator to freeze for 40 minutes, taken out, homogenized and dispersed evenly, and then ultrasonically extracted for 20 minutes, the temperature is controlled at 15±2°C, centrifuged for 10 minutes, and the speed is 8000rpm, collect the supernatant, and freeze-dry to obtain fresh Cordyceps sinensis extract.

Embodiment 3

[0042]Take 10g of fresh Cordyceps sinensis, cut it into pieces, add 12 times the amount of water, homogenate at high speed for 8 minutes, the homogenization speed is 16000rpm, control the temperature at 25±2°C, crush the fresh Cordyceps sinensis into fine particle suspension, ultrasonically assist extraction for 40 minutes, control The temperature is 25±2°C, and then the fresh Cordyceps sinensis extract is placed in a -20°C refrigerator to freeze for 60 minutes, taken out, homogenized and dispersed evenly, and then ultrasonically extracted for 40 minutes, the temperature is controlled at 25±2°C, centrifuged for 15 minutes, and the speed is 10000rpm, collect the supernatant, and freeze-dry to obtain fresh Cordyceps sinensis extract.

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Abstract

The invention discloses fresh Cordyceps extract with anti-melanoma activity, preparation method thereof and application thereof in the antitumor field. The fresh Cordyceps extract is prepared via the steps of shearing fresh Cordyceps, adding water, homogenizing, subjected homogenized fresh Cordyceps to low-temperature ultrasonic assisted extraction, centrifuging, collecting supernate, and lyophilizing to obtain the fresh Cordyceps extract. The fresh Cordyceps extract prepared herein has good inhibitory effect for melanoma cells, is high in safety and has good medicinal prospect. The preparation process of the extract herein is simple, and the extract is easy to produce on large scale and safe to use.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine and natural medicine, in particular to a preparation method of fresh Cordyceps sinensis extract and its anti-tumor application. Background technique [0002] Cordyceps sinensis is a complex of the subunit and larval corpse of the fungus Cordyceps sinensis (Berk.) Sacc. of the family Ergotaceae, which parasitizes on the larvae of bat moths. Cordyceps sinensis contains a variety of active ingredients, including nucleosides, polysaccharides, sterols, fatty acids, proteins, polypeptides and volatile components, etc., which have anti-tumor, immune system regulation, promotion of body cell damage repair, anti-free radicals, anti-oxidation, anti-aging , Protect myocardial and vascular cells, improve cardiovascular function, protect liver, protect kidney, regulate respiratory system, regulate blood system and regulate blood lipids and other functions. Many consumers use high-temperature stewin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/068A61P35/00
CPCA61K36/068A61K2236/331
Inventor 田野肖瑛江晓梅黄嘉仪徐香琴李文佳钱正明
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD