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Fluid sample processing device for biochemical reaction

A biochemical reaction, fluid sample technology, applied in the field of fluid processing devices, can solve problems such as complex fluid devices and processing procedures

Active Publication Date: 2017-02-22
XIAN TIANLONG SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, automating these processes requires complex fluidic devices and processing procedures, so there is still a need to develop such devices

Method used

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  • Fluid sample processing device for biochemical reaction
  • Fluid sample processing device for biochemical reaction
  • Fluid sample processing device for biochemical reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Such as figure 1 The fluid processing device shown is used for processing fluid samples, performing nucleic acid sample processing, extraction and purification, amplification, and fluorescent PCR detection, and can complete the qualitative detection of various nucleic acid targets in the sample.

[0072] The device of this embodiment includes a sample port 1 for adding fluid samples and a sample processing chamber 3 for fluid sample lysis and nucleic acid adsorption, the sample port 1 and sample processing chamber 3 communicate through a flow channel 2; flow channel 2 The outlet of the outlet is higher than the liquid level of the sample processing chamber 3 in the direction of fluid gravity, and the fluid sample enters the sample inlet 1 and reaches the sample processing chamber 3 through the flow channel 2 .

[0073] The sample processing chamber 3 is used to complete fluid sample lysis and nucleic acid adsorption. The first reagent chamber 4 is pre-packaged with a ly...

Embodiment 2

[0085] This embodiment differs from Embodiment 1, such as figure 2 As shown, the angle θ between the main channel 22 and each side channel 25 is equal to 90 degrees, the inlet of the channel 22 is connected with the second reaction chamber 16, the outlet of the channel 22 is the inlet of the side channel 25, and the outlet of the side channel 25 is The outlet communicates with the liquid separation chamber 21 . In this embodiment, the second reaction chamber 16 is connected and pressurized so that the eluent containing nucleic acid enters the liquid separation chamber 21 through the flow channel 22 and the side flow channel 25 for liquid separation.

Embodiment 3

[0087] This embodiment differs from embodiment 1 in that, as image 3 As shown, the liquid separation chambers 21 in the liquid separation detection chamber array are distributed on the main channel 22 . In this embodiment, the array of liquid separation chambers communicates with the second reaction chamber 16 through the main channel 22, and the second reaction chamber 16 is pressurized so that the nucleic acid eluent enters the array of liquid separation chambers through the main channel 22 for liquid separation.

[0088] Each detection chamber 23 is pre-packaged with PCR solid reagents for nucleic acid amplification and fluorescence detection, which are used to realize functions such as PCR reaction system establishment, nucleic acid amplification, and fluorescence detection under the cooperation of external thermal cycles and optical detection devices. Each detection chamber 23 communicates with the liquid separation chamber array 21 through the flow channel array 24, and...

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Abstract

The invention relates to a fluid sample processing device for a biochemical reaction. The device comprises a sample adding port, a sample processing chamber, at least two reaction chambers, a waste liquid chamber and a liquid separation detection chamber array; a fluid sample successively flows through the sample adding port, a sample processing chamber and at least two reaction chambers and then flows into the liquid separation detection chamber array or flows into the liquid separation detection chamber array and the waste liquid chamber by pressure and gravity; the sample processing chamber communicates with at least one reagent chamber, and each reaction chamber communicates with at least one reagent chamber. The device drives the liquid to flow by pressure and gravity, and relatively easily allows the fluid to smoothly enter and pass through the reaction chambers and the detection chambers. At the same time, the device can store a reagent in a form of vacuolar pre-encapsulation, can prevent the device from being additionally provided with a reagent storage structure, and reduces the total size of the device.

Description

technical field [0001] The invention relates to a fluid processing device for biochemical reactions, in particular to a device and method for processing, separating, extracting, distributing and biochemically reacting fluid biological, clinical or environmental samples. Background technique [0002] The identification and analysis of fluid samples, such as in laboratory testing, clinical testing, or environmental monitoring, typically involves a series of biochemical processing steps that may include chemical, optical, electronic, mechanical, thermal, or acoustic manipulation of the fluid sample . Whether involving batch processing workstations, benchtop instruments, or single-use cartridge-based POCT devices, such processing typically involves complex fluidics and handling procedures. [0003] One may have different purposes for analyzing fluid samples such as biological samples, clinical samples or environmental samples, one aspect of which is to develop a method that can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/686C12Q2563/107C12Q2565/629
Inventor 苗保刚李政彭年才李明田春孙瑶
Owner XIAN TIANLONG SCI & TECH
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