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Bacterial strain for reducing water-soluble sugar in tobacco leaves, screening method, cultivation method and application

A water-soluble sugar and bacterial strain technology, applied in the field of microbial strains, can solve the problems of sugar-base ratio imbalance and poor quality of tobacco leaves, and achieve the effects of weakening the acidity of smoke, improving sensory quality, and vigorous mycelium growth

Active Publication Date: 2019-08-09
CHINA TOBACCO SICHUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of abnormal Wickham yeast (Wickerhamomycesanomalus) that can reduce the water-soluble sugar in tobacco leaves to solve the problems of high content of water-soluble sugar in some tobacco leaves, serious imbalance of sugar-base ratio, and poor quality of tobacco leaves.

Method used

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  • Bacterial strain for reducing water-soluble sugar in tobacco leaves, screening method, cultivation method and application
  • Bacterial strain for reducing water-soluble sugar in tobacco leaves, screening method, cultivation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Screening and isolation of abnormal Wickham yeast:

[0035] Take 10 g of soil samples (Jiangzhou Tobacco Field Soil, Liangshan, Sichuan) and put them in 90 mL enrichment medium, and culture them at 30°C for 96 hours. If any hyphae grow during the culture process, pick them out and discard them. Take 1 mL of the enriched culture solution, put it into a petri dish containing the separation medium that was melted and cooled to 50 °C, mix evenly, and after solidification, place it in a 30 °C constant temperature incubator for 48 hours. The grown colony was picked and inserted into the acclimatization medium, cultured at 30°C for 48 hours, and the culture was diluted and spread on the separation medium plate to obtain a single colony, which was isolated and purified by streaking to obtain Wickerhamomyces anomalus).

[0036] The enrichment medium is: 20.0g glucose, 10.0g peptone, 5g yeast extract, 5g lactic acid, pH 5.0, sterilized at 121°C for 20min,

[0037] Th...

Embodiment 2

[0044] Example 2: Preparation of seed solution of abnormal Wickham yeast:

[0045] The Y05 colony of Wickhamiana abnormalis obtained in Example 1 was expanded and produced to obtain Bacillus cereus (code Y15) seed solution.

[0046] Slant culture: inoculate the frozen-preserved strain of Wickerhamomyces anomalus Y05 on the bran juice slant medium, and culture at a constant temperature of 30° C. for 1-2 days. The bran juice slant medium is: add 150 mL of water to 25 g of bran, heat and boil for 30 minutes, filter, make up 100 mL of the filtrate, add 2 g of glucose, 0.5 g of yeast extract, and 1.5 g of agar.

[0047]Shake flask liquid culture: 1000mL of 1% glucose aqueous solution, sterilized by high pressure steam, inoculate the bacteria obtained from slant culture into the medium liquid, culture temperature: 30°C, shaker speed 140-160r / min, shake flask culture 18- 24h, cultivate until the number of bacteria reaches 10 10 Above, the seed liquid is obtained.

Embodiment 3

[0049] The abnormal Wickham yeast seed solution obtained in Example 2 was added to tobacco leaves with a water content of 15% according to 30% of the weight of the tobacco leaves, and fermented at 30°C for 6 days. Compared with the unfermented blank tobacco leaves, the fermented tobacco leaves The total water-soluble sugar content in the medium was reduced by 60.5%, and the sensory evaluation results showed that the smoke coordination of the fermented tobacco leaves was better, the acidity was weakened, the sweetness returned, and the sense of satisfaction was enhanced.

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Abstract

The invention discloses a bacterial strain for reducing water-soluble sugar in tobacco leaves. The bacterial strain is Hansenula anomaly, and the bacterial strain is obtained by the following method: taking 9-10 g of soil samples in 90 mL enrichment medium, and then 28-32 Cultivate at ℃ for 92-100 hours to obtain the enriched culture medium. If any mycelium grows during the cultivation process, pick it out and discard it; Separate the petri dish of the culture medium, put it in a constant temperature incubator at 28-32°C and cultivate it for 45-50 hours after solidification; then select the grown colonies and insert them into the acclimatization medium, and culture them statically at a temperature of 28-32°C for 45-50 hours ; Then spread the culture gradient dilution on the separation medium plate to obtain a single colony, which is isolated and purified by streaking. Using the bacterial strain to ferment and treat tobacco leaves with high sugar content and serious imbalance of sugar-base ratio can reduce the water-soluble content by 20-95%.

Description

technical field [0001] The invention relates to a microbial strain, in particular to an abnormal Wickham yeast (Wickerhamomyces anomalus) capable of reducing water-soluble sugar in tobacco leaves, a screening method, a culture method and the application of the strain in tobacco leaves. Background technique [0002] Tobacco leaves are the raw material for cigarette production, and the quality of tobacco leaves is directly related to the quality of cigarettes. The chemical conventional components of tobacco leaves are mainly: total plant alkaloids, water-soluble total sugars, reducing sugars, total nitrogen, chlorine and potassium, and also contain macromolecular compounds such as protein, starch, cellulose, pectin, etc. The content of these components in tobacco leaves And the proportional relationship between certain components has an important impact on the quality of tobacco leaves. The water-soluble sugar in tobacco leaves includes reducing sugar and non-reducing water-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A24B15/20C12R1/645
CPCA24B15/20C12N1/16C12N1/145C12R2001/645
Inventor 周容李东亮寇明钰
Owner CHINA TOBACCO SICHUAN IND CO LTD
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