Rhodotorula mucilaginosa and application thereof in fermentation production of sea red astaxanthin
A technology of Rhodotorula japonicus and astaxanthin, which is applied in the field of microorganisms, can solve the problems of not meeting market demands and high prices, and achieve good application prospects, economic benefits, high yield, and strong growth and reproduction
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Embodiment 1
[0023]This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 25° C., and the cultivation time is 60 h. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 15 g / L, and the pH is adjusted to 5.5. The seed culture temperature is 25°C, the culture time is 24h h, the shaker speed is 140r / min, the composition of the seed medium is: glucose 10g / L, yeast powder 2g / L, sodium chloride 6g / L, and the remaining ingredients are water, pH5 .5.
[0024] According to the inoculum amount of 2% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 25°C, pH 5.5, 120...
Embodiment 2
[0026] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 33° C., and the cultivation time is 76 hours. The composition of the slant medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 20 g / L, and the pH is adjusted to 7.0. The seed culture temperature is 30°C, the culture time is 36h, the shaker speed is 160r / min, the seed medium composition is: glucose 12g / L, yeast powder 3g / L, sodium chloride 9g / L, and the remaining components are water, pH7. 0.
[0027] According to the inoculation amount of 10% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured at 33°C, pH 7.0, and 160 rpm for 76 hour...
Embodiment 3
[0029] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 26° C., and the cultivation time is 65 hours. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 16 g / L, and the pH is adjusted to 5.8. The seed culture temperature is 26°C, the culture time is 28h, the shaker speed is 150r / min, the composition of the seed medium is: glucose 10.5g / L, yeast powder 2.3g / L, sodium chloride 7g / L, and the rest is water. pH5.8.
[0030] According to the inoculation amount of 3% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 28°C, pH 6.0, 130rpm for 6...
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