Rhodotorula mucilaginosa and application thereof in fermentation production of sea red astaxanthin

A technology of Rhodotorula japonicus and astaxanthin, which is applied in the field of microorganisms, can solve the problems of not meeting market demands and high prices, and achieve good application prospects, economic benefits, high yield, and strong growth and reproduction

Inactive Publication Date: 2015-08-12
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many biotechnology companies in the United States, Canada, the European Union and other countries are working on the development and production of such products, but they are far from meeting market demand, so the price is relatively high

Method used

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  • Rhodotorula mucilaginosa and application thereof in fermentation production of sea red astaxanthin
  • Rhodotorula mucilaginosa and application thereof in fermentation production of sea red astaxanthin

Examples

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Embodiment 1

[0023]This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 25° C., and the cultivation time is 60 h. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 15 g / L, and the pH is adjusted to 5.5. The seed culture temperature is 25°C, the culture time is 24h h, the shaker speed is 140r / min, the composition of the seed medium is: glucose 10g / L, yeast powder 2g / L, sodium chloride 6g / L, and the remaining ingredients are water, pH5 .5.

[0024] According to the inoculum amount of 2% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 25°C, pH 5.5, 120...

Embodiment 2

[0026] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 33° C., and the cultivation time is 76 hours. The composition of the slant medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 20 g / L, and the pH is adjusted to 7.0. The seed culture temperature is 30°C, the culture time is 36h, the shaker speed is 160r / min, the seed medium composition is: glucose 12g / L, yeast powder 3g / L, sodium chloride 9g / L, and the remaining components are water, pH7. 0.

[0027] According to the inoculation amount of 10% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured at 33°C, pH 7.0, and 160 rpm for 76 hour...

Embodiment 3

[0029] This embodiment provides the application of Rhodotorula gelatin in the present invention in the fermentation production of sea red astaxanthin, which is realized through the following steps: the described Rhodotorula gelatin is cultured on a slant and seed cultured successively to obtain activated seed liquid; wherein, The slant cultivation temperature is 26° C., and the cultivation time is 65 hours. The composition of the slant culture medium is: malt extract powder 130.1 g / L, distilled water 1000 g / L, agar 16 g / L, and the pH is adjusted to 5.8. The seed culture temperature is 26°C, the culture time is 28h, the shaker speed is 150r / min, the composition of the seed medium is: glucose 10.5g / L, yeast powder 2.3g / L, sodium chloride 7g / L, and the rest is water. pH5.8.

[0030] According to the inoculation amount of 3% by volume, the seed solution was inoculated into the fermentation medium containing sea red astaxanthin, and cultured by shaking at 28°C, pH 6.0, 130rpm for 6...

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Abstract

The invention discloses rhodotorula mucilaginosa GH101 and application thereof in fermentation production of sea red astaxanthin. The application comprises the following steps: sequentially carrying out slant culture and seed culture on rhodotorula mucilaginosa, so as to obtain activated seed liquid; inoculating the seed liquid into a fermentation culture medium containing sea red astaxanthin according to an inoculation quantity of 2%-10% by weight, and carrying out shaking culture for 60-76 hours at the conditions that the temperature is 25-33 DEG C, the pH is 5.5-7.0, and the rotation speed is 120rpm-160rpm, so as to obtain a rhodotorula mucilaginosa thallus containing the sea red astaxanthin. Rhodotorula mucilaginosa provided by the invention can be applied to the fermentation production of the sea red astaxanthin and is utilized for producing the sea red astaxanthin and repeatedly screening produced strains to obtain a strain with strong growth fecundity and relatively high yield of the sea red astaxanthin, and a good application prospect is provided for the industrial production of astaxanthin.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of rhodotorula colloides and its application in fermenting and producing sea red astaxanthin. Background technique [0002] Sea red astaxanthin is a kind of carotenoid with strong anti-oxidation ability and biological coloring ability. It has been widely used in food, medicine, cosmetics, feed and many other fields. It has high application value in preventing cardiovascular and cerebrovascular diseases, protecting eyes and central nervous system, preventing ultraviolet radiation, inhibiting tumors, anti-inflammation, and relieving exercise fatigue. Sea red astaxanthin, a carotenoid with high antioxidant capacity, its mobility is close to natural astaxanthin ester in thin layer chromatography, and its antioxidant capacity is also close to natural L-astaxanthin, so it is named, DPPH Its antioxidant capacity is about 20% to 40% stronger than that of natural L-astaxanthin....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P23/00
CPCC12P23/00C12N1/145C12R2001/645
Inventor 魏升宁张文丽孙常明程振伟张美丽李纪丰
Owner WEIHAI LIDA BIOTECH
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