Royal jelly polypeptide and application thereof
A technology of royal jelly and queen bee larvae, applied in the field of biomedicine, can solve the problems of difficult screening of biologically active polypeptides, unsuitable for royal jelly proteolysis, and few polypeptides
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Embodiment 1
[0060] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) at 1:1, grind it into a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 20,000×g for 20 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 20000×g at 4°C for 20 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.
[0061] Take part of the intestinal enzyme solution, and use the BCA method to ...
Embodiment 2
[0066] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) at 1:1, grind it into a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 18000×g for 30 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 18000×g at 4°C for 30 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.
[0067] Take part of the intestinal enzyme solution, and use the BCA method to d...
Embodiment 3
[0072] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7.2 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) being 1:1.5, it was ground into a homogenate with an electric homogenizer under ice bath conditions. Centrifuge at 25,000×g for 24 minutes at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of the suspended solution, the lower layer of sedimentation, and the recovery of the middle layer. The middle layer was centrifuged again at 25000×g at 4°C for 24 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.
[0073] Take part of the intestinal enzyme solution, and...
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