Application of superparamagnetic nano-particles serving as marker in preparation of PJI diagnosis test paper

A superparamagnetic and nanoparticle technology, applied in the field of infection diagnosis around joint prostheses, can solve the problems that ELISA detection technology is not very economical and fast, cannot be directly applied in clinical practice, and the test cycle is long, so as to achieve simple and easy diagnosis and realize quantitative High detection and high sensitivity

Inactive Publication Date: 2017-02-22
GENERAL HOSPITAL OF PLA
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Problems solved by technology

Although ELISA is widely used in clinical diagnostic testing, a set of test ELISA kits (96-well plate) is expensive, about several thousand yuan
However, PJI is not a high incidence rate. The results reported by each center are pre-retained joint fluid samples. After a certain number of samples are collected, they will be tested uniformly. The test cycle is very long and requires coating, sample addition, enzyme-label

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  • Application of superparamagnetic nano-particles serving as marker in preparation of PJI diagnosis test paper

Examples

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Example Embodiment

[0019] Example:

[0020] This example provides the application of superparamagnetic nanoparticles as markers in the preparation of PJI diagnostic test paper. In this example, superparamagnetic nanoparticles were used to replace the colloidal gold in the existing colloidal gold immunochromatography technology as a marker. Specifically, this embodiment provides an immunochromatographic test paper for PJI diagnosis, in which superparamagnetic nanoparticles are used as markers instead of colloidal gold.

[0021] In a further improvement of this example, the antibody-conjugated complex of superparamagnetic nanoparticles and α-defensin was first prepared. The specific preparation method was: weigh 50 μg of antibody to α-defensin and 100 μL of superparamagnetic nanoparticles Mix and couple, react on a shaker at 120 rpm at 37°C for 3 hours, and then block with 5% BSA for 30 minutes. The obtained conjugated complex of superparamagnetic nanoparticles and α-defensin antibody can be sto...

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Abstract

The invention belongs to the technical field of joint prosthesis peripheral infection diagnosis and particularly provides application of superparamagnetic nano-particles serving as a marker in preparation of PJI diagnosis test paper. The superparamagnetic nano-particles replace colloidal gold to serve as the marker, and a novel magnetic immunochromatography technology is formed. The application has the advantages that the PJI diagnosis test paper provided through the application is high in sensitivity and specificity and short in detection time, and detection is convenient; on the aspect of qualitative detection, the qualitative detection limit can be lower than 20 ng/mL, and the detection time is shorter than 10 min; on the aspect of quantitative detection, the quantitative detection limit is lower than 10 ng/mL, and the detection time is shorter than 20 min.

Description

technical field [0001] The invention belongs to the technical field of infection diagnosis around joint prostheses, and in particular relates to the application of superparamagnetic nanoparticles as markers in the preparation of PJI diagnostic test paper. Background technique [0002] Periprosthetic Joint Infection (PJI) is considered to be a catastrophic complication after artificial joint replacement. It has been reported in the literature that PJI occurs in about 1% to 3% of patients after joint replacement surgery. As the incidence of PJI increases, it imposes a huge economic burden on both the patient and the healthcare system. Early diagnosis of PJI is of great significance for the treatment of PJI. However, there is still no sensitive, accurate, fast and easy method to diagnose periprosthetic infection. [0003] Currently, the commonly used diagnostic criteria for MSIS are mainly based on clinical manifestations, laboratory examinations of peripheral blood and joint...

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Application Information

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IPC IPC(8): G01N33/543G01N33/68
CPCG01N33/5434G01N33/54346G01N33/68
Inventor 陈继营沈研丁芳付君李睿刘侃
Owner GENERAL HOSPITAL OF PLA
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