Application of superparamagnetic nano-particles serving as marker in preparation of PJI diagnosis test paper

Abstract

The invention belongs to the technical field of joint prosthesis peripheral infection diagnosis and particularly provides application of superparamagnetic nano-particles serving as a marker in preparation of PJI diagnosis test paper. The superparamagnetic nano-particles replace colloidal gold to serve as the marker, and a novel magnetic immunochromatography technology is formed. The application has the advantages that the PJI diagnosis test paper provided through the application is high in sensitivity and specificity and short in detection time, and detection is convenient; on the aspect of qualitative detection, the qualitative detection limit can be lower than 20 ng / mL, and the detection time is shorter than 10 min; on the aspect of quantitative detection, the quantitative detection limit is lower than 10 ng / mL, and the detection time is shorter than 20 min.

Description

technical field

[0001] The invention belongs to the technical field of infection diagnosis around joint prostheses, and in particular relates to the application of superparamagnetic nanoparticles as markers in the preparation of PJI diagnostic test paper. Background technique

[0002] Periprosthetic Joint Infection (PJI) is considered to be a catastrophic complication after artificial joint replacement. It has been reported in the literature that PJI occurs in about 1% to 3% of patients after joint replacement surgery. As the incidence of PJI increases, it imposes a huge economic burden on both the patient and the healthcare system. Early diagnosis of PJI is of great significance for the treatment of PJI. However, there is still no sensitive, accurate, fast and easy method to diagnose periprosthetic infection.

[0003] Currently, the commonly used diagnostic criteria for MSIS are mainly based on clinical manifestations, laboratory examinations of peripheral blood and joint...

Images