Method for rapidly culturing and producing galanthamine, lycorine and lycoramine by rapid propagation of bulblets of lycoris

A technology of galantamine and lycoris bulbs, which is applied in the field of lycoris, which produces galantamine and lycorine through the small bulbs of lycoris, can solve the problems of large market gap for lycoris plants, and achieve rich raw materials , Occupy a small space, alleviate the effect of endangered and seriously deficient

Inactive Publication Date: 2017-03-29
LIAOCHENG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem that there is a relatively large gap in the market of lycoris plants for extracting raw materials of galantamine, lycorine, and lycorine, the purpose of the present invention is to provide a method for producing galantamine, lycorine, and lycoris through small bulbs of lycoris in China. Sensitive method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for producing galantamine, lycorine, and ricolamine by using Chinese lycoris bulbs, comprising the following steps:

[0043] A) Selection and sterilization of explants: the bulbs of 'Chinese Lycoris' were used as explants, soaked and rinsed with 2% detergent solution, shaken at 100 rpm for 15 minutes, rinsed with water, and 75% alcohol disinfection for 30 seconds, 0.1% mercuric chloride sterilization treatment, immersion sterilization for 10 minutes, rinse with sterile water 6 times, and set aside;

[0044] B, explant inoculation: the explant prepared in step A is inoculated in the SH medium containing 6-BA 5.0 mg / L and 0.5 mg / LNAA, 2,4-D 2.0 mg / L, Among them, 30g / L sucrose, 7.5g / L agar powder, pH5.8; 8 hours of light and 16 hours of darkness every day.

[0045] C. Induction of bulblets: the explants obtained in step B are cultivated in the SH medium of 6-BA 5.0 mg / L and 1.0 mg / L NAA BA, wherein sucrose 40g / L, agar powder 7.5g / L L, pH5.80, 12 hours of light a...

Embodiment 2

[0050]A method for producing galantamine, lycorine, and ricolamine by using Chinese lycoris bulbs, comprising the following steps:

[0051] A) Selection and sterilization of explants: the bulbs of 'Chinese Lycoris' were used as explants, soaked and rinsed with 3% detergent solution, shaken at 120 rpm for 25 minutes, rinsed with water, and 75% alcohol disinfection for 30 seconds, 0.1% mercuric chloride sterilization treatment, soaking for 11 minutes, rinsing with sterile water for 8 times, standby;

[0052] B, explant inoculation: the explant prepared in step A is inoculated in SH medium containing 6-BA 5.0 mg / L and 0.5 mg / LNAA, 2,4-D 1.0 mg / L, Among them, 30g / L sucrose, 7.5g / L agar powder, pH5.8; 8 hours of light and 16 hours of darkness every day.

[0053] C. Induction of bulblets: the explants obtained in step B are cultivated in the SH medium of 6-BA 5.0 mg / L and 1.0 mg / L NAA BA, wherein sucrose 50g / L, agar powder 7.5g / L L, pH5.85, 12 hours of light and 12 hours of darkne...

Embodiment 3

[0058] A method for producing galantamine, lycorine, and ricolamine by using Chinese lycoris bulbs, comprising the following steps:

[0059] A) Selection and sterilization of explants: the bulbs of 'Chinese Lycoris' were used as explants, soaked and rinsed with 3% detergent solution, shaken at 120 rpm for 30 minutes, rinsed with water, and then rinsed with water. 75% alcohol disinfection for 30 seconds, 0.1% mercuric chloride sterilization treatment, soaking for 12 minutes, rinsing with sterile water for 8 times, spare;

[0060] B, explant inoculation: the explant prepared in step A is inoculated in the SH medium containing 6-BA 5.0 mg / L and 1.0 mg / LNAA, 2,4-D 1.0 mg / L, Among them, 30g / L sucrose, 7.5g / L agar powder, pH5.90; 8 hours of light and 16 hours of darkness every day.

[0061] C. Induction of bulblets: the explants obtained in step B are cultivated in the SH medium of 6-BA 5.0 mg / L and 1.0 mg / L NAA BA, wherein sucrose 40g / L, agar powder 7.5g / L L, pH5.90, 12 hours of ...

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Abstract

The invention provides a method for rapidly culturing and producing galanthamine, lycorine and lycoramine by rapid propagation of bulblets of lycoris. The method comprises the following steps: taking a bulb of 'lycoris chinensis' as an explant, sterilizing, inoculating and culturing for a period of time, generating bulblets at a wound of a scale, and continuing carrying out multiplication culture on the bulblets; and drying tissues of the bulblets of the lycoris chinensis, grinding and ultrasonically extracting with methanol for three times to obtain a methanol solution containing the galanthamine, the lycorine and the lycoramine. The problems that resources of lycoris herbs are in shortage, and chemical synthesis of the galanthamine, the lycorine and the lycoramine is complicated are solved by a tissue culturing technology. According to a used basic medium, an SH medium is used as a base, and ingredients such as 6-benzylamino adenine, alpha-naphthylacetic acid and a yeast extract are used as auxiliary ingredients; and the method lays a foundation for industrial production of the galanthamine, the lycorine and the lycoramine and is environment-friendly, raw materials are abundant, and the supply gap of markets to the galanthamine, the lycorine and the lycoramine can be relieved well.

Description

technical field [0001] The invention relates to the production of galantamine, lycorine and lycorine, in particular to a method for producing galantamine, lycorine and lycorine from Chinese lycoris bulbs. Background technique [0002] Galanthamine, also known as Galanthamine, has a chemical formula of C 17 h 21 NO 3， The molecular weight is 287.36. White or light yellow crystalline powder. Odorless, bitter taste. The melting point is 127-129°C. Soluble in ethanol, acetone, chloroform, insoluble in benzene, ether, water. Usually, the bulbs of Amaryllidaceae (Amaryllidaceae) are used as raw materials, and they are repeatedly extracted and refined with ethanol and chloroform under different acidities. Galantamine hydrobromide is used for myasthenia gravis, progressive muscular dystrophy, sequelae of poliomyelitis, children with cerebral palsy, sensory or movement disorders caused by nervous system diseases, and multiple neuritis. The oral dosage form of this drug can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 穆红梅
Owner LIAOCHENG UNIV
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