Analysis of Arabidopsis thaliana root tip cell structure using three-dimensional recombinant imaging technology

Abstract

The invention discloses a method of using a three dimensional reconstruction imaging technology to analyze the structure of root tip cells of arabidopis thaliana, and belongs to the field of cell structure analysis. According to the method, roots of arabidopis thaliana are subjected to a horizontal freezing slicing treatment to obtain root cross sections, which only contain the root caps, the thickness of the slice is only 10 [mu]m, and thus the influence of the resolution ratio of the Z axis of a microscope on the imaging reality is greatly reduced; by optimizing the conditions of microscopic photography, the shooting stepping is minimized to less than 0.4 [mu]m, the Pinhole value is reduced so as to ensure that the thickness of a calculated optical slice is less than 0.8 [mu]m, the fineness of the photos is guaranteed; the scanning range comprises the whole structure of a stele cell from top to bottom; a finer microscopic imaging picture can be obtained, the influence of stray light of non-focal planes on the images is effectively reduced, the image resolution ratio is improved, the structure of a studied object can be realistically represented; and by using the provided three dimensional reconstruction imaging technology, the intra-cell endoplasmic reticulum structure can be determined more directly and accurately.

Description

technical field [0001] The invention relates to a method for overall plant single cell optical microscopy imaging, in particular to a method for three-dimensional overall imaging of plant single cell, which belongs to the field of cell structure analysis. Background technique [0002] Arabidopsis thaliana, also known as Arabidopsis thaliana, Arabidopsis, Arabian grass. It belongs to the Angiosperm Phylum, Dicotyledoneae, and Brassicaceae. Its genome is about 125 million base pairs and 5 pairs of chromosomes, which is the smallest known plant genome. Arabidopsis is a self-pollinating plant with high gene homozygosity. It has a high mutation rate when treated with physical and chemical factors, and it is easy to obtain defective types of various metabolic functions. Due to the above advantages, Arabidopsis thaliana is a good material for genetic research. It is known as "the fruit fly in plants" by scientists. It is a widely used model organism. There are more than 6,000 labo...

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Problems solved by technology

However, because the root tips of Arabidopsis seedlings are very small, the general microscopic imaging of root tip cells can only be observed directly under the optical microscope, and the observation is the longitudinal optical section of the root, so the results obtained are inaccurate (because there are relative neighbor cell occlusion problem)

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