A strain of Schwannella desulfurum and its application
A Beschwanella, sulfide technology, applied in the field of biology and environmental protection, can solve the problems of difficult to obtain, slow desulfurization rate, slow growth and so on
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Embodiment 1
[0030] Embodiment 1: strain screening
[0031] Take an appropriate amount of sludge from the aerobic aeration tank of Chengdu No. 2 Sewage Treatment Plant, and add it into a reactor containing 5L sodium thiosulfate medium at an inoculum size of 20%. Enrichment culture at ℃ for 7 days, repeat 3 to 4 times, use sodium sulfide medium for plate separation, after 3 to 4 days, colonies are formed, pick a single colony and insert it into Na 2 S.9H 2 O medium culture, repeated more than 4 times to obtain pure bacteria.
Embodiment 2
[0032] Embodiment 2: bacterial strain identification
[0033] The strains were inoculated in sodium thiosulfate liquid medium according to the same inoculum size, placed in shakers at different temperatures (20°C, 25°C, 30°C, 35°C, 40°C) and cultured for 30 hours. It can grow within ~35°C, and its optimum growth temperature is 30°C; take initial pH values of 5.0, 6.0, 7.0, 8.0, and 9.0 and culture it for 30 hours at the optimum growth temperature, and confirm that the bacteria can grow within 5.0 ~ 9.0. The optimum growth pH is 7.0;
[0034] The isolated and purified bacterial strain was observed under a scanning electron microscope (SEM), and the results were as follows: figure 1 As shown, the bacterium is short rod-shaped, 0.6-1.2 μm long, 0.2-0.6 μm wide, Gram-negative, with flagella; its colony is round, with a raised surface, and the color is light yellow;
[0035] Bacterial Whole Genome Rapid Extraction Kit was used to extract the whole genome of the pure strain, PCR...
Embodiment 3
[0037] Embodiment 3: desulfurization bottle test
[0038] 200ml sodium sulfide medium solution (600mg / L S 2- ) was sterilized under ultraviolet light for 30 minutes, put into a sterilized 500ml Erlenmeyer flask under aseptic conditions, then add 50ml of Schwannella strain cultured for 30h, stopper the bottle mouth with an aerobic stopper, and place in a shaker Oscillate in the bed (30°C, 200r / min), measure the initial sulfide concentration to be 536.57mg / L S 2- Add the same amount of sterile water to the control group, set three repetitions in each experimental group, sample and measure the sulfide concentration and elemental sulfur concentration every 30 minutes, and the results are averaged. After 5 hours, its measurement and calculation results are as attached image 3 shown. After the desulfurization bacteria were added to the sodium sulfide solution of the experimental group, the sulfide concentration was significantly lower than that of the control group, and the conv...
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