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Rooting induction method for tissue culture seedling of cork oak

A technology of Quercus variabilis and Quercus variabilis, applied in the field of rooting induction of Quercus cork tissue culture seedlings, can solve the problems of not thick roots, small number of roots, uneven rooting, etc., and achieve shortened rooting cycle, fast rooting speed, and many roots Effect

Inactive Publication Date: 2017-05-10
LIUZHOU LINGTONG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture, the problems of low rooting rate, inconsistent rooting, small number of roots and not strong root system often appear.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select subcultured buds of Quercus cork that have been cultured for 35-36 days in conventional tissue culture. After disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select the subcultured buds that grow robustly and have a height of 1-2 cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.0mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0023] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2000lux, and light 16h / d for rooting culture. ...

Embodiment 2

[0026] Select the secondary buds of Quercus cork that have been cultured for 36-37 days in the conventional tissue culture, and after disinfecting the surface of the bottle, select the vigorous growth of the secondary bud clusters with a height of 1-2 cm in the sterile space on the ultra-clean workbench. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 1.5mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0027] After 30-35 days of pre-rooting culture for single buds, transfer the single buds to the rooting medium and place them under the conditions of temperature 20±1°C, humidity 40%, light intensity 2500lux, and light 15h / d for ro...

Embodiment 3

[0030] Select the secondary buds of Quercus cork that have been cultured for 37-38 days in the conventional tissue culture, and after disinfecting the surface of the bottle, in the aseptic space on the ultra-clean workbench, select the vigorous growth of the secondary buds, with a height of 1-2 cm. Single buds, cut at 2-3mm below the node, and remove the base leaves and petioles. The pruned single buds were inoculated in the pre-rooting medium, and placed under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for pre-rooting culture. The raw material content of the pre-rooting medium described therein is: 1 / 2 improved MS medium+NAA 2.0mg / L+activated carbon 6g / L++VC 15mg / L+sucrose 25g / L+agar 5.0g / L.

[0031] After 30-35 days of pre-rooting culture for single buds, transfer the single buds into the rooting medium, and place them under the conditions of temperature 20±1°C, humidity 45%, light intensity 2000lux, and light 16h / d for rooti...

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PUM

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Abstract

The invention discloses a rooting induction method for a tissue culture seedling of cork oak. The rooting induction method comprises the steps of selecting cork oak proliferation buds which are subjected to strong bud culture for 35-40 days in a conventional tissue culture process, trimming the cork oak proliferation buds, inoculating the cork oak proliferation buds into a pre-rooting culture medium, carrying out pre-rooting culture for 30-35 days, and transferring the cork oak proliferation buds into a rooting culture medium for culturing. The rooting induction method has the beneficial effects that the rooting cycle of the cork oak proliferation buds is shortened, the rooting rate is high, root systems are well developed with high quality, the transplanting survival rate of rooted tissue culture seedlings is high, tissue culture industrialized seedling of the cork oak can be realized, high-quality seedlings are provided for the construction of artificial clonal forests; and the method has relatively high economic, social and ecological benefits.

Description

technical field [0001] The invention relates to the vegetative propagation technology of oak cork, in particular to a method for inducing rooting of quercus cork tissue culture seedlings. Background technique [0002] cork oak ( Quercus variabilis Bl ) Also known as Cork Quercus, Quercus vulgaris, and Quercus vulgaris, it is a tree of the genus Quercus in the family Fagaceae, with a broad oval crown, many trunks, taupe, deep longitudinal fissures, and a thick cork layer. The branchlets are light brown, the male inflorescences are born in the lower part of the current year's branches, and the female flowers are solitary or twin in the axils of the current year's branches. The flowering period is May; the fruit matures in September-October of the following year. It is an important timber tree species in China. Cork oak likes light and is often born on sunny slopes in mountains, but it is better for young trees to have side shade. Strong adaptability to climate and soil. I...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 黄文卫
Owner LIUZHOU LINGTONG TECH CO LTD