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Preparation method and application of green plum extract

A technology of extract and green plum, which is applied in the field of preparation of green plum extract, can solve the problems of difficult industrialization, less development, and imperfect preparation methods, and achieve excellent antibacterial and anti-aging effects, easy absorption, and mild texture

Inactive Publication Date: 2017-05-10
BIOLAND BIOCHEM HAIMEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although greengage products have huge potential value in beauty, the greengage products in my country are mainly concentrated in food, and are made into preserved fruits, greengage wine, and greengage juice products. There are very few developments in cosmetics related products, and the preparation methods are not very good. Perfect, industrialization is more difficult

Method used

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  • Preparation method and application of green plum extract
  • Preparation method and application of green plum extract
  • Preparation method and application of green plum extract

Examples

Experimental program
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Effect test

preparation example Construction

[0019] The preparation method of plum extract in the present invention comprises the following steps:

[0020] Step 1, take fresh greengage fruit, wash and remove the core, and squeeze it into juice with a juicer;

[0021] Step 2, extract the juice with 10-20 times the solvent at room temperature for 7-12 hours, the solvent is deionized water or one of 1,3 butanediol, propylene glycol, and dipropylene glycol, and then microwave extraction at 40-60°C 25-45min, use a filter membrane with a relative molecular weight of 1000-2000 to remove macromolecular impurities such as protein, colloid, and limiter to obtain a filtrate;

[0022] Step 3, decolorize the primary filtrate with 1%-2% activated carbon for 20-40 minutes, and obtain the secondary filtrate after filtering;

[0023] Step 4, the secondary filtrate is concentrated under reduced pressure at a temperature of 60-70° C. to 1 / 3-1 / 5 volume, and the filtrate after vacuum filtration is the green plum extract.

Embodiment 1

[0026] Inhibition experiment of 10% green plum extract solution on common bacteria:

[0027] The bacterial solution preparation table is shown in Table 1 below:

[0028]

[0029] Table 1

[0030] (1) Add Escherichia coli, Staphylococcus aureus, Bacillus aeruginosa, and Candida albicans at 1%, add Aspergillus niger at 10%, mix well, and treat at 27°C for 1 day;

[0031] (2) Dilute the 0.85%NaCl group with 0.85% NaCl by 10^1, 10^2, 10^3, 10^4 times, take 0.01ml and spot on the corresponding medium, culture upside down at 27°C for 1 day, count For the number of colonies, the dilution factor of the number of colonies between 10-50 is used as the experimental dilution factor, and the dilution factor is determined to be 10^4 times;

[0032] (3) Dilute the sample bacterial solution treated for 1 day to the experimental dilution factor of 4, take 0.01ml each and spot on the corresponding medium, culture at 27°C for 1 day, observe the growth of the colony, and count the number of ...

Embodiment 2

[0037] 10% green plum extract solution for free radical scavenging ability test:

[0038] (1) 3.943mgDPPH was diluted to 50mL with 95% ethanol; 10mg vitamin E was diluted to 100mL with 95% ethanol; 10% green plum extract solution;

[0039] (2) UV zero calibration, 95% ethanol as a blank sample, the configuration table is shown in Table 3 below;

[0040] (3) 500μL DPPH+1000μL 95% ethanol, shake for 10s, let stand for 10min, measure the absorbance value twice, as shown in Table 4 below.

[0041]

[0042] table 3

[0043]

[0044] Table 4

[0045] recombine figure 1 It can be seen that the green plum extract has a better effect on scavenging DPPH free radicals, with an IC50 value of 10000 μg / mL, and the higher the content of green plum extract, the stronger the ability to scavenge free radicals.

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Abstract

The invention discloses a preparation method of green plum extract; the preparation method is highly operable, and the green plum extract can effectively enrich the substances in green plum, such as maleic acid and furfural, maximally retain polyphenols and flavones therein, and help inhibit the growth of germs and fungi on skin surface and reduce oxygenated free radicals generated by skin cells, and accordingly has excellent antibacterial anti-aging effect; the green plum extract is mild in nature, easy to absorb and non-irritating, meets the requirement of people for safety and effectiveness of cosmetics, and has a promising application prospect.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method and application of green plum extract. Background technique [0002] Greengage is a plant of the genus Cherry in the family Rosaceae, which has been cultivated in my country for more than two thousand years. Green plum has long been used for sterilization, treatment of hepatitis and extraction of traditional Chinese medicine dipterocarp in Hainan, and it has been officially included in the list of "both food and medicine" by the Ministry of Health. [0003] Research at home and abroad shows that green plum fruit is rich in citric acid and malic acid, which can relieve fatigue, promote skin cell metabolism, promote hormone secretion and activation, beautify skin, and delay aging. [0004] Although greengage products have huge potential value in beauty, the greengage products in my country are mainly concentrated in food, and are made into preser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61Q17/00A61P31/04A61P31/10A61P17/18
CPCA61K8/97A61K2800/805A61Q17/005A61Q19/08
Inventor 金基洙姚永斌张玉娟卢勇金荣熙金庚泰陈秀玉刘培玉
Owner BIOLAND BIOCHEM HAIMEN CO LTD
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