Quantitative detection method of heparin
A quantitative detection method and heparin technology, which can be used in measurement devices, electrochemical variables of materials, instruments, etc., can solve the problems of low sensitivity, high cost, and inability to provide, and achieve the effect of high sensitivity and low cost.
Active Publication Date: 2017-05-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology
Traditional blood heparin concentration detection methods (such as aPTT technology, anti-Xa assay or thrombus viscometer (TEG)) have some difficulties, such as high cost, low sensitivity, and some cannot provide accurate quantitative information, etc.
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[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.
[0030] This case lists the quantitative detection method of heparin of an embodiment, specifically may include:
[0031] 1) A gold electrode was used as the working electrode in the experiment. Electrodes need to be cleaned in newly configured piranha washing solution (98% H 2 SO 4 : 30%H 2 o 2 =3:1) for 5 minutes to remove impurities adsorbed on the electrode surface.
[0032] 2) After washing with distilled water, polish the electrode with silicon carbide sandpaper (3000 mesh) until the mirror surface is smooth. Then, the electrodes were ultrasonically cleaned in ethanol and distilled water for 5 minutes each. Next, it was dried in a nitrogen atmosphere before use.
[0033] 3) Treated electrode with 0.5M H 2 SO 4 solution for electrochemical cleaning. Then, the el...
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Abstract
The invention relates to a quantitative detection method of heparin. The quantitative detection method comprises the following steps: 1) taking a gold electrode as a working electrode and pre-treating the gold electrode; 2) modifying a polypeptide on the surface of the gold electrode; 3) modifying mercapto hexanol on the surface of the gold electrode and occupying the surface of a gold electrode which is not modified by the polypeptide; 4) inserting the gold electrode into a solution to be detected and reacting; and collecting a corresponding electrochemical impedance spectrum and calculating a resistance value; and 5) obtaining the concentration of the heparin in the solution to be detected according to a corresponding relation between the resistance value and the concentration of the heparin, wherein the polypeptide sequence is CGSGRKRLQVQLSIRT. The quantitative detection method of the heparin, provided by the invention, has the characteristics of high sensitivity, good specificity, anti-interference property and low cost and the detection limit is 0.01mu g / ml; and the designed specific polypeptide is combined with an electrochemical technology so that application of heparin biological sensing can be realized.
Description
technical field [0001] The invention relates to a quantitative detection method for heparin, in particular to an electrochemical detection method for heparin based on specific polypeptide modification. Background technique [0002] Heparin is a naturally occurring biomacromolecule containing polyanions with high charge density, and is the most structurally complex member of the glycosaminoglycan (GAG) family. Heparin acts as an intravenous anticoagulant in the blood coagulation cascade by interacting with thrombin inhibitors such as antithrombin III (ATIII). Therefore, heparin is considered as a prophylactic and therapeutic agent widely used in clinics, especially as an anticoagulant medically used in surgery. Traditional blood heparin concentration detection methods (such as aPTT technology, anti-Xa assay or thrombus viscometer (TEG)) have some difficulties, such as high cost, low sensitivity, and some cannot provide accurate quantitative information, etc. [0003] In rec...
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IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3275
Inventor 缪鹏孟凡渝梁伟国孙海旋王弼陡杨大威
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI