Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of plant induction medium for regeneration of callus from leaves of T.

A technology for inducing medium and regenerating plants, which is applied in the field of plant regenerating plant inducing medium and can solve the problems of low reproduction coefficient and low differentiation rate of cultured plants, etc.

Active Publication Date: 2018-11-16
FORESTRY RES INST OF HEILONGJIANG PROVINCE
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of low natural reproduction coefficient of Cypripedium grandiflorum and low differentiation rate of plants cultivated in the existing regeneration induction medium, and provides a plant induction medium for regeneration of Cypripedium grandiflorum leaves

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0019] Specific implementation mode one: this implementation mode obtains the regenerated plant of Cypripedium grandiflorum blade:

[0020] 1. Collection and processing of leaves of Cypripedium grandiflora

[0021] Cypripedium grandiflorum was collected from Jiangshanjiao Experimental Forest Farm in Heilongjiang Province. The female parent is a perennial herb with strong growth; the type of explants is the leaves of the current year, and the collection season is mid-to-early May during the growing season of Cypripedium grandiflorum, and the collection time is selected on a sunny day It will be held from 9 to 10 in the morning. Store the collected leaves in a refrigerator for later use.

[0022] 2. Disinfection method

[0023]Take out the leaves refrigerated at low temperature, first wash the leaves with detergent to remove the surface dust, then rinse with water, then put them on the ultra-clean workbench and sterilize them with 75% ethanol for 10 seconds, then rinse them wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cypripedium macranthum Sw.f. leaf regeneration plant induction medium and relates to a regeneration plant induction medium for plants. The problems that the cypripedium macranthum Sw.f. has a low natural reproduction coefficient and the existing regeneration induction medium is low in cultured plant differentiation rate are solved. The ingredients of the cypripedium macranthum Sw.f. leaf regeneration plant induction medium comprise macroelement ingredients, trace element ingredients and organic matter ingredients, and the induction medium has a pH value of 5.5-6.0. When young leaves of cypripedium macranthum Sw.f. are subjected to regeneration plant induction culture by utilizing the cypripedium macranthum Sw.f. leaf regeneration plant induction medium disclosed by the invention, the young leaves of cypripedium macranthum are easily induced to form callus on the induction and differentiation medium disclosed by the invention, and the callus induction rate can reach 90% or higher; and moreover, the induced light green callus is transferred onto a subculture medium in the invention for performing subculture, the probability of inducing the callus into plants is high, and the regeneration plant induction rate can reach 70%.

Description

technical field [0001] The invention relates to a plant regeneration induction medium. Background technique [0002] Cypripedium macranthum Sw.f., a rare wild flower, is an important variety of Cypripedium. It is a perennial plant and is distributed in northeast my country. Plant height 35-50cm, thick rhizome, 3-6 cauline leaves, wide oval. Flowers are large, 3-5cm long, fragrant, solitary on the top of the stem. The pedicels are slightly curved, and the flowers are pure white. The lips are like women's shoes, so they are also called shoe orchids. Cypripedium large white flower can emit fragrance, and the flower shape is strange, which has a very high ornamental value. Cypripedium grandiflorum grows in the understory, forest edge or grassy slopes with rich humus and good drainage at an altitude of 400-2400 meters. It is distributed in China's Heilongjiang, Jilin, Liaoning, Inner Mongolia, Shandong, Taiwan, Japan, the Korean Peninsula and Russia. Because it is a wild fl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 孙一萌刘延滨李晶李秀芬牟兆军王晓蓉张华崔崧魏嵩蒙宽宏宫主王承义
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com