Guided tissue regeneration membrane and preparation method thereof

A technology for guiding tissue regeneration and membrane precursors, which can be used in the field of medical materials to solve problems such as excessive degradation in vivo and poor mechanical strength.

Active Publication Date: 2017-05-24
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is the main component of hoof tissue. As a membrane to guide tissue regeneration, it has bioactivity and biocompatibility unmatched by other

Method used

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  • Guided tissue regeneration membrane and preparation method thereof
  • Guided tissue regeneration membrane and preparation method thereof
  • Guided tissue regeneration membrane and preparation method thereof

Examples

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preparation example Construction

[0027] Such as figure 1 The preparation method of the guided tissue regeneration film includes the following steps:

[0028] S10, pretreating the skin of the mammal to obtain a dermis slice.

[0029] The operation of obtaining the dermis skin piece after pretreatment of the mammalian skin is as follows: the mammalian skin is preliminarily degreased and depilated by a mechanical method, and then the mammalian skin is removed with a skin removal machine to remove 0.1mm to 0.4mm. After the epidermis and subcutaneous fat tissue, a dermis slice with a thickness of 0.2 mm to 1 mm was made.

[0030] Generally, the dermis skin can be cut into skins with a size of 3cm×6cm~5cm~10cm.

[0031] Mammalian skin consists of successive layers of fur, epidermis, dermis, and subcutaneous tissue.

[0032] Specifically, the skin of mammals is the skin of pigs, cattle, sheep or horses. Preferably, the mammalian skin is belly skin of pigs, cows, sheep or horses.

[0033] The obtained dermis sli...

Embodiment 1

[0059] (1) Preparation of dermal slices

[0060] Preliminarily degrease the whole abdominal skin of healthy pigs by mechanical method, remove the hair with tweezers, remove the subcutaneous fat of the pigskin with an electric skin remover, and then remove 0.4mm of the epidermis to make a dermis with a thickness of 0.3mm The skin slices were then soaked in an aqueous solution of penicillin-streptomycin with a mass concentration of 0.01%, placed in a refrigerator at 4°C overnight, and then transferred to a low-temperature refrigerator at -25°C for storage.

[0061] (2) Preparation of membrane precursor for guided tissue regeneration

[0062] Decellularization treatment: Soak the thawed dermis slices successively in 0.1% sodium chloride (hypotonic salt) and 1.0% sodium chloride (hypertonic salt) solutions for 2 times, 6h / time , through the treatment of hypotonic solution, the tissue absorbs water and swells, so that the cells in the tissue are broken, and the collagen fiber stru...

Embodiment 2

[0070] (1) Preparation of dermal slices

[0071] Preliminarily degrease the whole abdominal skin of healthy pigs by mechanical method, remove the hair with tweezers, remove the subcutaneous tissue of the pigskin with an electric skin remover, and then remove 0.1mm of the epidermis to make a dermis with a thickness of 0.9mm The skin slices were then soaked in an aqueous solution of gentamicin with a mass concentration of 0.1%, placed in a refrigerator at 4°C overnight, and then transferred to a low-temperature refrigerator at -25°C for storage.

[0072] (2) Preparation of membrane precursor for guided tissue regeneration

[0073] Decellularization treatment: soak the thawed dermis slices successively in a solution with a mass concentration of 0.9% sodium chloride (hypotonic salt) and a mass concentration of 9.0% sodium chloride (hypertonic salt) for 3 times, 4h / time , through the treatment of hypotonic solution, the tissue absorbs water and swells, so that the cells in the tissu...

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Abstract

The invention discloses a guided tissue regeneration membrane and a preparation method thereof. The preparation method comprises the steps of pretreating mammalian skin to obtain corium layer skin graft; sequentially performing decellularized treatment, virus inactivation treatment and dense shrinkage treatment on the corium layer skin graft to obtain a guided tissue regeneration membrane precursor; arranging the guided tissue regeneration membrane precursor in solution of soluble strontium salt in a mode of making the rough surface upward and making the smooth surface downward to perform activation, transferring the precursor to simulated body fluid to perform mineralization, and performing precooling and freeze drying to obtain the guided tissue regeneration membrane. According to the method for preparing the guided tissue regeneration membrane, by performing decellularized treatment, virus inactivation treatment and dense shrinkage treatment on the corium layer skin graft, the obtained guided tissue regeneration membrane precursor has the rough surface, the rough surface is mineralized in the simulated body fluid after being compounded with strontium element, and the obtained guided tissue regeneration membrane can slowly release Sr<2+>and Ca<2+> to peripheral defective bone tissues and has good bone induction and bone conduction functions.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a membrane for guiding tissue regeneration and a preparation method thereof. Background technique [0002] Guided Tissue Regeneration (GTR) is currently the most advanced treatment method that can effectively treat periodontal disease. The key technology is to use the biological barrier membrane, that is, the spatial isolation of the tissue regeneration guiding membrane, to prevent the gingival epithelium and Tissue cells migrate to the root surface, and at the same time selectively make periodontal cells and alveolar bone cells adhere to the root surface and differentiate to form new periodontal attachments. Therefore, the role of the guiding membrane is crucial. With the clinical application of GTR technology more and more widely, the type and performance of the guide membrane have become the key factors for its development. [0003] The guide membranes used in traditional cl...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/14
Inventor 李丽花朱勇军钟梅玲佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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