Polybrene microwell plate pre-transfusion detection method
A detection technology and microplate technology, applied in the field of pre-transfusion detection of polybrene microplates
Inactive Publication Date: 2017-05-24
重庆市涪陵三海兰陵有限责任公司
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Abstract
The invention relates to an improved artificial polybrene microwell plate pre-transfusion detection method. The improved artificial polybrene microwell plate pre-transfusion detection method comprises following characteristics: 1, consumable items and auxiliary facilities are changed, self-designed microwell plates (represented by formula 1) are adopted, and no test tube or centrifugal machine is used; and 2, the concentration and the amount of polybrene reagent are changed. Polybrene is a four amine polymer, is capable of neutralizing negative charges on erythrocyte, reducing Zeta electric potential, and shortening the distance between cells. When the concentration of polybrene is low, and the distance between erythrocytes is reduced, reversible nonspecific erythrocyte condensation is caused via centrifugation; when the concentration of polybrene is increased, nonspecific erythrocyte condensation is caused directly. Then the surfaces of erythrocytes are negatively charged, erythrocyte condensation disappears because of negative charge repelling among erythrocytes, and erythrocyte condensation is caused by erythrocyte antigens and antibodies, and the affinity of antigens and antibodies doesn't fade away.
Application Domain
Biological testing
Technology Topic
Erythrocyte antigenChemistry +10
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