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Polybrene microwell plate pre-transfusion detection method

A detection technology and microplate technology, applied in the field of pre-transfusion detection of polybrene microplates

Inactive Publication Date: 2017-05-24
重庆市涪陵三海兰陵有限责任公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the reaction time of this method is 1 hour, and at the same time, a blood type instrument is required for interpretation

Method used

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  • Polybrene microwell plate pre-transfusion detection method
  • Polybrene microwell plate pre-transfusion detection method
  • Polybrene microwell plate pre-transfusion detection method

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Embodiment Construction

[0020] Referring to Fig. 1-4, the present invention first changes the used consumables and supporting facilities by improving the manual polybrene method, and uses self-designed microporous plates ( figure 1 ), no need for test tubes and centrifuges; second, change the concentration and amount of polybrene reagent. Polybrene is a tetramine polymer that can neutralize the negative charge on red blood cells, reduce the Zeta potential, and shorten the distance between cells. At low concentrations, when the distance between red blood cells is shortened, a reversible Non-specific erythrocyte aggregation phenomenon; when the concentration of polybrene increases, erythrocytes directly form non-specific erythrocyte aggregation. When the negative charge on the surface of red blood cells is restored, due to the repulsion of negative charges between red blood cells, the phenomenon of red blood cell aggregation disappears, but the real agglutination caused by red blood cell antigen antib...

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Abstract

The invention relates to an improved artificial polybrene microwell plate pre-transfusion detection method. The improved artificial polybrene microwell plate pre-transfusion detection method comprises following characteristics: 1, consumable items and auxiliary facilities are changed, self-designed microwell plates (represented by formula 1) are adopted, and no test tube or centrifugal machine is used; and 2, the concentration and the amount of polybrene reagent are changed. Polybrene is a four amine polymer, is capable of neutralizing negative charges on erythrocyte, reducing Zeta electric potential, and shortening the distance between cells. When the concentration of polybrene is low, and the distance between erythrocytes is reduced, reversible nonspecific erythrocyte condensation is caused via centrifugation; when the concentration of polybrene is increased, nonspecific erythrocyte condensation is caused directly. Then the surfaces of erythrocytes are negatively charged, erythrocyte condensation disappears because of negative charge repelling among erythrocytes, and erythrocyte condensation is caused by erythrocyte antigens and antibodies, and the affinity of antigens and antibodies doesn't fade away.

Description

technical field [0001] The invention relates to the field of pre-blood transfusion inspection, in particular to a polybrene microporous plate pre-blood transfusion detection technology. Background technique [0002] The traditional polybrene method is to use a test tube to detect through steps such as centrifugation. This method requires supporting centrifuges and other instruments. However, at the scene of an emergency or trauma, due to the critical condition of the patient, it takes a while to collect the specimen and send it to the laboratory for cross-matching, which delays the timing of the patient's rescue. In addition, in relatively remote areas and countries, due to lack of resources and no supporting equipment such as centrifuges, it is difficult to ensure the desired effect of pre-transfusion inspections. Compared with other blood matching methods, the manual polybrene method has the advantages of sensitivity, accuracy, simple operation, and rapidity (only 3 to ...

Claims

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Application Information

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IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 盛小玲张维佳
Owner 重庆市涪陵三海兰陵有限责任公司
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