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Technique for detection by using polybrene microplate before blood transfusion

A detection technology and microplate technology, applied in the field of pre-transfusion detection of polybrene microplate

Inactive Publication Date: 2010-02-10
刘景春 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction time of this method is 1 hour, and at the same time, a blood type instrument is required for interpretation

Method used

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  • Technique for detection by using polybrene microplate before blood transfusion
  • Technique for detection by using polybrene microplate before blood transfusion
  • Technique for detection by using polybrene microplate before blood transfusion

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Embodiment Construction

[0020] Referring to Fig. 1-4, the present invention first changes the used consumables and supporting facilities by improving the manual polybrene method, and uses self-designed microporous plates ( figure 1 ), no need for test tubes and centrifuges; second, change the concentration and amount of polybrene reagent. Polybrene is a tetramine polymer that can neutralize the negative charge on red blood cells, reduce the Zeta potential, and shorten the distance between cells. At low concentrations, when the distance between red blood cells is shortened, a reversible Non-specific erythrocyte aggregation phenomenon; when the concentration of polybrene increases, erythrocytes directly form non-specific erythrocyte aggregation. When the negative charge on the surface of red blood cells is restored, due to the repulsion of negative charges between red blood cells, the phenomenon of red blood cell aggregation disappears, but the real agglutination caused by red blood cell antigen antib...

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Abstract

The invention relates to a technique for detection by using an improved polybrene microplate before blood transfusion. In the technique, the designed microplate (figure 1) is used instead of used consumable items and supporting facility and no test tube nor centrifuge is used, and on the other hand, the concentration and dosage of a polybrene reagent are changed. The polybrene is a tetraamine polymer which can neutralize negative electric charges on red blood cells, reduce the Zeta electric potential and shorten the distance between cells. Under the condition of a low concentration, a reversible nonspecific hemagglutination phenomenon can appear when the distance between the red blood cells is shortened; and when the concentration of the polybrene is increased, the red blood cells directlyform nonspecific hemagglutination. When the negative electric charges on the surfaces of the red blood cells are restored, the hemagglutination phenomenon disappears because of the repulsion of the negative electric charges among the red blood cells, but the affinity or the agglutination, really caused by the antigen antibodies of the red blood cells, of the antigen antibodies does not disappear.

Description

technical field [0001] The invention relates to the field of pre-blood transfusion inspection, in particular to a polybrene microporous plate pre-blood transfusion detection technology. Background technique [0002] The traditional polybrene method is to use a test tube to detect through steps such as centrifugation. This method requires supporting centrifuges and other instruments. However, at the scene of an emergency or trauma, due to the critical condition of the patient, it takes a while to collect the specimen and send it to the laboratory for cross-matching, which delays the timing of the patient's rescue. In addition, in relatively remote areas and countries, due to lack of resources and no supporting equipment such as centrifuges, it is difficult to ensure the desired effect of pre-transfusion inspections. Compared with other blood matching methods, the manual polybrene method has the advantages of sensitivity, accuracy, simple operation, and rapidity (only 3 to 5 ...

Claims

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Application Information

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IPC IPC(8): G01N33/80
Inventor 刘景春车家林刘赴平
Owner 刘景春
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