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Cancer related gene expression difference detection kit and applications thereof

A gene expression difference detection kit technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. Accurate results and high sample throughput

Active Publication Date: 2017-05-31
SHANG OUTDO BIOTECH CO LTD
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  • Claims
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Problems solved by technology

[0005] The invention proposes a cancer-related gene expression difference detection kit and its application, which solves the technical problems of low throughput or poor sensitivity and accuracy in the prior art

Method used

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  • Cancer related gene expression difference detection kit and applications thereof
  • Cancer related gene expression difference detection kit and applications thereof

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Embodiment 1

[0031] A kit for detecting differences in expression of cancer-related genes, comprising: cDNA templates and housekeeping gene primers. The preparation of the cDNA template is as follows: extract the RNA of the patient's cancer and paracancerous tissues; add genomic DNase to the RNA to digest the remaining genomic DNA, and collect the total RNA after purification; then, perform quality control on the purified RNA. Detect, select high-quality RNA, and use RNA reverse transcriptase to synthesize cDNA. Finally, after the cDNA of each sample was accurately quantified, it was uniformly added to a 96-well plate for PCR according to the principle of equal amount per well, and was freeze-dried to prepare a cDNA template. The housekeeping gene primers are a pair of forward and reverse primers for the housekeeping gene β-actin. Specifically, the forward and reverse primers of β-actin can be selected as the nucleotide chain of the sequence shown in SEQ ID NO: 1-2 or its complementary ch...

Embodiment 2

[0044] The application of the cancer-related gene expression difference detection kit is specifically the detection method of the kit, which includes the following steps:

[0045] (1) Preparation of cDNA template.

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Abstract

The invention provides a cancer related gene expression difference detection kit and applications thereof. The detection kit comprises a cDNA template, which is obtained through reverse transcription of RNA extracted from human cancer tissues and corresponding para-carcinoma tissues; and housekeeping gene primers, namely a pair of forward primer and reverse primer of housekeeping gene gamma-actin. The invention also discloses a detection method of the detection kit. The detection kit has the characteristics of high sample throughput, quick and accurate detection, and high sensitivity; and can rapidly, accurately, and sensitively detect the expression situation of a target gene in a large amount of cancer samples. On the basis of clinical information and follow-up information, the researchers can carry out various related statistical analysis.

Description

technical field [0001] The invention relates to the technical field of kits and their applications, in particular to a cancer-related gene expression differential detection kit and its applications. Background technique [0002] In recent years, cancer and cardiovascular system diseases, respiratory system diseases and diabetes have been ranked among the top deadly diseases, and the incidence of cancer has also shown an upward trend in the past decade. According to statistics from the National Cancer Center, in 2015, there were 4.2916 million cancer cases in China and 2.8142 million deaths. Lung cancer and gastric cancer ranked the top two in cancer incidence and death in the country. The incidence of all tumors was slightly stable between 2000 and 2011 in men (0.2% annual increase) and more pronounced in women (2.2% annual increase). At present, according to the prognosis of various cancers, it can be roughly divided into three categories: the first category, early treatme...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6886C12Q2600/158C12Q2521/101C12Q2545/101C12Q2563/107
Inventor 亓垚
Owner SHANG OUTDO BIOTECH CO LTD
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