Structure illumination super-resolution microscopy imaging system and imaging method thereof
A technology of structured light illumination and microscopic imaging, which is applied in the direction of material analysis, analysis of materials, fluorescence/phosphorescence, etc. by optical means, which can solve the problems of failure to provide iteration, small amount of calculation, sacrifice of speed and accuracy, etc.
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[0041] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.
[0042] Such as figure 1 As shown, the structured light illumination super-resolution microscopy imaging system of this embodiment includes: an illumination light source 1, a rotating structured light generator 2, a rotating device 3, a first converging lens 4, a dichroic mirror 6, an objective lens 7, and an object Stage 8, sample, second converging lens 9 and digital imaging device 10; wherein, the rotating structured light generator 2 adopts a grating, is arranged on the rotating device 3, takes the optical axis as the rotating axis, and the rotating device drives the grating to rotate 360°, The rotation angle is not fixed; the illumination source 1 emits excitation light; the excitation light is diffracted by the grating; the diffracted excitation light is converged by the first converging lens 4, transmitted by the dichroic mirror 6, f...
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