Cordyceps militaris strain screening and mutagenesis method capable of producing heat-resistant proteinase and cordycepin at high yield

Abstract

The invention belongs to the field of microbial fungi, and relates to a Cordyceps militaris strain capable of producing heat-resistant proteinase and cordycepin at high yield and application thereof. The strain is prepared by the following steps: selecting a Cordyceps militaris wild-type strain newly separated from the natural world as the initial strain, carrying out composite mutagenization under the actions of Cordyceps militaris protoplast nitrosoguanidine and ultraviolet light, separating and screening to obtain the strain. The strain was collected by China General Microbiological Culture Collection Center on November 11th, 2016; the collection number is CGMCC No.13179; the strain is named HYCM12; and the classification designation is Cordyceps militaris. After the strain is subjected to deep liquid ventilation fermentation, the cordycepin content reaches 2.89 g / L, and the heat-resistant proteinase activity reaches 5326U / ml. Meanwhile, the method is simple to operate and easy to implement, has the advantages of short period, abundant and accessible raw material sources, low cost, no environmental pollution, low equipment and reagent price and the like, and is convenient for large-scale production.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for screening and mutagenesis of Cordyceps militaris strains with high yield of heat-resistant protease and cordycepin. Background technique [0002] Cordyceps militaris[ Cordyceps militaris (L.) Link], also known as Cordyceps militaris, modern scientific research shows that Cordyceps militaris is rich in active substances, such as Cordyceps polysaccharides, cordycepin, cordycepic acid, amino acids, proteases, etc., with anti-tumor, anti-oxidation and aging, anti-aging Anti-inflammatory, anti-fatigue, anti-virus growth, enhancing immunity and other pharmacological effects and nourishing functions, it is a great medicinal treasure with development potential. Among the active ingredients of Cordyceps militaris, cordycepin is the main active ingredient (especially nucleosides) in Cordyceps militaris, and it is the first nucleoside antibiotic to be isolated. Cordyce...

AI Technical Summary

Problems solved by technology

Cordyceps militaris is the main strain that produces cordycepin, but its fruiting body has a long growth cycle, low yield, and relatively low content of cordycepin. The cost of extracting cordycepin from fruiting bodies is extremely high and expensive, and it is difficult to meet the wide clinical use