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Application of the antisense nucleotide sequence of ubc9 in the preparation of drugs for inhibiting the growth of cancer cells

A technology of antisense nucleotides and cancer cells, applied in the field of tumor biotherapy, to achieve the effects of reducing the degree of malignancy, inhibiting growth, and reducing the number of

Active Publication Date: 2019-12-03
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the current situation of poor tumor treatment effect, the present invention provides an antisense nucleotide sequence of UBC9 to interfere with the expression of UBC9, thereby inhibiting the malignant phenotype of cells containing double microsomes and inhibiting the growth of malignant tumor cells.

Method used

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  • Application of the antisense nucleotide sequence of ubc9 in the preparation of drugs for inhibiting the growth of cancer cells
  • Application of the antisense nucleotide sequence of ubc9 in the preparation of drugs for inhibiting the growth of cancer cells
  • Application of the antisense nucleotide sequence of ubc9 in the preparation of drugs for inhibiting the growth of cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Relevant genes in the process of embodiment 1 SUMOylation are screened

[0065] In this example, the malignant tumor cell lines COLO320DM and UACC-1598-4 containing stable double-minions and the cell lines COLO320HSR and UACC-1598-59 without double-minions were selected as the research objects of the preliminary screening.

[0066] 1. Method

[0067] (1) Apply qRT-PCR to screen the related genes in the SUMOylation process:

[0068] ① Extraction of RNA

[0069] Add 1ml Trizol to the cells in the logarithmic growth phase, let them act on ice for 5 minutes, blow off the cells and suck them into a 1.5ml EP tube. Add 0.25ml of chloroform, shake vigorously about 30 times, let stand at room temperature for 5 minutes, centrifuge at 12000 rpm, 4°C for 15 minutes; absorb 0.5ml of the upper aqueous phase into a new EP tube, add an equal volume of isopropanol Precipitate RNA, mix gently, let stand at room temperature for 10 minutes, centrifuge at 12,000 rpm, 4°C for 10 minutes, ...

Embodiment 2

[0100] Example 2 Effect of UBC9 Antisense Nucleotide Sequence on Double Minor Number and Cell Function in Tumor Cells

[0101] 1. Method

[0102] The malignant tumor cell lines UACC-1598-4 cells and COLO320DM cells containing double microsomes were selected as the cell lines used in this example:

[0103] The expression level of UBC9 protein in COLO320DM and UACC-1598-4 cells containing more and stable double minutes was higher than that in COLO320HSR and UACC-1598-59 cells without double minutes, while the expression level of SAE2 was not. significant difference. Therefore, the UBC9 gene was selected as the target gene of this experiment, and UACC-1598-4 cells and COLO320DM cells were the cell lines used in this example.

[0104] RPMI-1640 medium containing 10% fetal bovine serum in 5% CO 2 Human ovarian cancer cell line UACC-1598-4 and human colorectal adenocarcinoma cell line COLO320DM were cultured in a constant temperature incubator at 37°C.

[0105] (1) Establishment...

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Abstract

The invention discloses an application of an antisense oligonucleotide sequence of UBC9 in preparation of a medicine for inhibiting cancer cell growth. A specific antisense oligonucleotide sequence of the UBC9 is designed and synthesized. By adopting the specific antisense oligonucleotide sequence of the UBC9, the expression level of the UBC9 in a malignant cell containing double minutes is reduced through an RNAi interference method and the quantity of double minutes is reduced, so that the targets of reducing the malignant degree of the tumor cell and effectively inhibiting malignant cell growth are achieved. A novel targeted therapy scheme is provided for biotherapy of the malignant cell containing the double minutes.

Description

technical field [0001] The present invention relates to a new application of the antisense nucleotide sequence of UBC9. Specifically, the present invention relates to the application of the antisense nucleotide sequence of NSMCE2 in the preparation of drugs for inhibiting cancer cell growth, which belongs to the technical field of tumor biotherapy. Background technique [0002] The human UBC9 gene (Ubiquitin-conjugating enzyme) is also known as UBE2I. The UBC9 gene is generally highly expressed in human tissues and organs, and its biological functions are different in different tissues and different developmental stages of the organism. The deletion of this gene is fatal to the organism. The deletion of UBC9 gene will lead to the decrease of the growth ability and invasion ability of tumor cells, the decrease of the overall SUMO level of the cells, the increase of the proportion of apoptotic cells, and the appearance of nuclear duplication and senescence of the cells. The s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7105A61K48/00A61P35/00
CPCA61K31/7105
Inventor 傅松滨吴杰孙文靖计薇贾学渊于旸邹宏岩白静
Owner HARBIN MEDICAL UNIVERSITY
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