High-efficiency tissue culturing and rapid propagating method of apple stock Qingzhen 1 seed tree

Abstract

The invention belongs to the field of plant cultivation and particular relates to a high-efficiency tissue culturing and rapid propagating method of apple stock Qingzhen 1 seed tree. The method comprises the steps of taking a material and performing explant sterilizing treatment; inoculating the material which is sterilely treated into a starting culture medium; choosing bud seedlings which grow identically in the starting culture medium and inoculating the bud seedlings into a multiplying medium; neatly arranging the bud seedlings into the same bottle in two rows in a mode that the abaxial sides contact the culture medium after subculture multiplication, performing dark culture treatment on inoculated blades, taking out and culturing in 16 / 8h light after treatment, choosing rootless tissue culture seedlings with identical growth vigor and inoculating into a rooting culture medium; transplanting the tissue culture seedlings which are rooted to a culture medium to be transplanted and domesticated. By means of the method, the final transplantation success rate is 80% or more.

Description

technical field [0001] The invention belongs to the field of plant propagation, and in particular relates to a method for establishing a high-efficiency tissue culture and rapid propagation system for apple rootstock Qingzhen No. 1 mother tree. Background technique [0002] Excellent apple rootstock is the basis for high-quality and high-yield apples. Tissue culture technology can effectively reduce apple diseases, and supply a large number of high-quality seedlings in a short time to meet market demand. The technology of tissue culture of Malus is relatively difficult, which affects the application of tissue culture seedlings in production and promotion. High-efficiency in vitro regeneration and rooting of tissue culture seedlings are key steps in tissue culture rapid propagation technology. Whether or not rooting and the quality of rooting directly affect the emergence of seedlings. The type and concentration ratio of auxin, the concentration of inorganic salts, and ligh...

AI Technical Summary

Problems solved by technology

The tissue culture technique of Malus plants is difficult, which affects the application of tissue culture seedlings in production and promotion

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