Unlock instant, AI-driven research and patent intelligence for your innovation.

Tissue culture and multiplication method of white dichotomanthes tristaniicarpa wood

A tissue culture and beef tendon technology, applied in the field of tissue culture and proliferation of white bull tendon wood, can solve the problems of low transplant survival rate, high cost of seedling raising, difficulty in rooting of beef tendon cuttings, etc., so as to improve seedling raising efficiency and reduce seedling raising cost effect

Inactive Publication Date: 2017-06-20
云南省农业科学院园艺作物研究所
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seedlings propagated by seeds have a well-developed main root, deep penetration into the soil, few fibrous roots, and are evergreen trees, so the survival rate of transplanting is low; cutting propagation requires a certain degree of humidity, temperature, air and self-growth from the outside world, as well as sufficient nutrition from the outside world. In addition to substances, some traces of special active substances are needed, and the operation is complicated. Due to the difficulty in rooting of beef tendon cuttings, the cost of raising seedlings is high, and the efficiency is low, which affects the speed of its promotion.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and multiplication method of white dichotomanthes tristaniicarpa wood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for tissue culture and multiplication of Albino chinensis, the concrete steps are as follows:

[0018] (1) Selection of explants: In the fine weather from March to May, cut the young stems or tips of the vegetative branches of the beef tendons;

[0019] (2) Sterilization of explants: Cut off the leaves on the tender stems, wash the stems or stem tips with detergent, rinse them with tap water, put the stems or stem tips into an ultra-clean workbench, and use 75 % alcohol for 30s, then rinse the alcohol with sterilized distilled water, put it into 0.1% mercuric chloride solution for 20 minutes, and then wash it with sterile water for 4 times;

[0020] (3) Establishment of the sterile line: the basic medium used for the establishment of the sterile line is MS medium, and the hormones used are 6BA and NAA. Put the medium into jars, each bottle is filled with 30 mL of medium, and after sealing, Carry out high-temperature and high-pressure sterilization, and cool do...

Embodiment 2

[0023] A method for tissue culture and multiplication of Albino chinensis, the concrete steps are as follows:

[0024] (1) Selection of explants: In the fine weather from March to May, cut the young stems or tips of the vegetative branches of the beef tendons;

[0025] (2) Sterilization of explants: Cut off the leaves on the tender stems, wash the stems or stem tips with detergent, rinse them with tap water, put the stems or stem tips into an ultra-clean workbench, and use 75 % alcohol for 30s, then rinse the alcohol with sterilized distilled water, put it into 0.1% mercuric chloride solution for 25 minutes, and then wash it with sterile water for 4 times;

[0026] (3) Establishment of the sterile line: the basic medium used for the establishment of the sterile line is MS medium, and the hormones used are 6BA and NAA. Put the medium into jars, each bottle is filled with 35 mL of medium, and after sealing, Carry out high-temperature and high-pressure sterilization, and cool do...

Embodiment 3

[0029] A method for tissue culture and multiplication of Albino chinensis, the concrete steps are as follows:

[0030] (1) Selection of explants: In the fine weather from March to May, cut the young stems or tips of the vegetative branches of the beef tendons;

[0031] (2) Sterilization of explants: Cut off the leaves on the tender stems, wash the stems or stem tips with detergent, rinse them with tap water, put the stems or stem tips into an ultra-clean workbench, and use 75 % alcohol for 30s, then rinse the alcohol with sterilized distilled water, put it into 0.1% mercuric acid solution for 30 minutes, and then wash it with sterile water for 4 times;

[0032] (3) Establishment of the sterile line: the basic medium used for the establishment of the sterile line is MS medium, and the hormones used are 6BA and NAA. Put the medium into jars with 40mL of medium in each bottle, and seal it. Carry out high-temperature and high-pressure sterilization, and cool down after sterilizat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture and multiplication method of white dichotomanthes tristaniicarpa wood. The tissue culture and multiplication method of the white dichotomanthes tristaniicarpa wood particularly comprises the following steps: selection of explants, sterilization of explants, establishment of an aseptic line and subculture multiplication, wherein the selection of the explants is to shear tender stem segments or stem tips of current grown nutritional branches of dichotomanthes tristaniicarpa in fine weather in March to May; and the sterilization of the explants comprises the steps of shearing leaves on the tender stems, cleaning the stem segments or the stem tips with detergent, completely washing with tap water, putting the stem segments or the stem tips into a bechtop, soaking with 75 percent alcohol for 30 seconds, completely washing away the alcohol with sterile distilled water, putting into 0.1 percent mercury bichloride solution and soaking for 20 to 30 minutes, and cleaning with sterile water for 4 times. By adoption of the tissue culture and multiplication method, foundation is laid for growing seedlings in batches, reducing seedling growing cost and improving seedling growing efficiency.

Description

technical field [0001] The invention relates to a method for multiplying trees, in particular to a method for tissue-cultured and multiplied wood. Background technique [0002] Beef tendons belong to the genus Beef Tendon in the Rosaceae in terms of plant classification, and there is only one species and one variety produced in Yunnan. The Institute of Horticultural Crops of the Yunnan Academy of Agricultural Sciences combined the reality of high mountains and steep slopes in Yunnan, drought in winter and spring, collected local materials, and conducted research on beef tendon plants on the basis of summarizing the experience of the masses. According to preliminary observations, whether grafted with apples or pears, beef tendon strips have obvious effects of dwarfing trees and early fruiting, and can be used for multiple purposes. It is a promising dwarfing stock for apples and pears. But beef tendon is an evergreen shrub, evergreen in all seasons, well-developed main root,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 李坤明陈伟陈瑶胡忠荣万萌
Owner 云南省农业科学院园艺作物研究所