Uniform speed symmetrical uniaxial tension cell mechanical device for real-time in-situ observation

Abstract

The invention discloses a uniform speed symmetrical uniaxial tension cell mechanical device for real-time in-situ observation. The device can realize uniform speed symmetrical uniaxial tension of cells culture on a transparent tension vessel, the location of the tension vessel middle part is constant in the tension process, and through coordination with temperature, humidity and carbon dioxide supply, real-time in-situ long-term observation for a few days on cells under a loading state can be realized. The device comprises: a power unit, a motion exeution unit, the transparent tension vessel, a clamping platform and a flexible telescopic air sac. The power unit achieves uniform speed symmetrical uniaxial tension of the transparent tension vessel through the motion exeution unit, the tension vessel is covered by the flexible telescopic air sac, and through environmental setting, the device realizes days of continuous culture and tensile loading of cells. The bottom surface of the clamping platform is processed into a size matching a microscope loading platform, and can be placed on the microscope loading platform stably. The device provided by the invention can realize uniform speed symmetrical uniaxial tension of the transparent tension vessel according to a set speed and tension length, and real-time in-situ long-term observation and multi-point observation.

Description

technical field [0001] The invention relates to a force loading device, in particular to a uniform-velocity symmetrical uniaxial tensile cell mechanics device capable of real-time on-site observation, belonging to the fields of biomechanics and mechanobiology. Background technique [0002] Cells are the basic unit of life, and the movement and growth of cells cannot do without the participation of mechanical stimuli. With the in-depth research on mechanical regulation of cell growth, division, migration, apoptosis, etc., it is found that mechanical stimulation can significantly affect the individual and group behavior of cells, from microscopic protein expression, signal transduction, to the arrangement of subcellular structures, From cell division, proliferation, differentiation, apoptosis, alignment, polarization, migration, wound healing at the cell scale to tissue morphology and formation at the macro scale. [0003] Existing devices for stretching and loading cells, in...

AI Technical Summary

Problems solved by technology

[0003] Existing devices for stretching and loading cells, in addition to optical tweezers and magnetic tweezers for stretching single cells, many of them stretch the basement membrane to which cells are attached, such as by vacuuming and under the membrane. Cooperate with the geometric entities to achieve uniaxial or quasi-biaxial stretching. The geometric entities and the bottom plate under this device are opaque and cannot be observed in real time.

There are also some devices where one end of the basement membrane is fixed and the other end moves. The basement membrane of these devices is deforming all the time, there is no real-time observation position, and the device is not compact enough

There are also some devices that use cam transmission to stretch the base film, but the stretching speed is not uniform, and the stretching length depends on the geometry of the cam, and the adjustable range is narrow

All of the above devices cannot achieve long-term cultivation and real-time on-site observation on the microscope

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