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Method for detecting residual clenbuterol in pork

A detection method, clenbuterol technology, applied in the field of fluorescent nanomaterials, can solve life-threatening problems

Inactive Publication Date: 2017-06-20
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, later studies have shown that clenbuterol is very easy to accumulate in livestock and poultry. When people eat meat products with clenbuterol residues for a long time, adverse reactions such as headache, chest tightness, heart palpitations, and numbness of limbs will occur. can be life threatening

Method used

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  • Method for detecting residual clenbuterol in pork
  • Method for detecting residual clenbuterol in pork
  • Method for detecting residual clenbuterol in pork

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Embodiment 1

[0021] A method for detecting residual clenbuterol in pork, the specific operation steps are as follows:

[0022] Gold nanocluster preparation, 10 mL, 50 mg / mL BSA was kept at 37 °C for 10 min, added 10 mL, 10 mM chloroauric acid aqueous solution and magnetically stirred for 2 min, then quickly added 1 mL of 1 M sodium hydroxide solution, The mixture was continuously stirred at 37°C for 12 h. The prepared crude gold nanoclusters were dialyzed with water for 24 h; unreacted bovine serum albumin was removed with a G-75 gel molecular sieve column, and the gold nanocluster powder was obtained after freeze-drying.

[0023] Dilute hydrochloric acid to 0.10 mol / L aqueous solution, and then react with 0.10 mol / L sodium nitrite aqueous solution at a ratio of 1:1 to generate nitrous acid, which is unstable, and quickly added to different concentrations of clenbuterol hydrochloride In the solution, the diazotized derivative of clenbuterol hydrochloride was obtained.

[0024] Add differ...

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Abstract

The invention relates to a method for detecting residual clenbuterol in pork. The method adopts a gold nano-cluster as a fluorescent probe to interact with clenbuterol after the diazotization reaction, so that the content of the clenbuterol remaining in the pork is detected. The method provided by the invention can accurately and rapidly detect the clenbuterol. The method is simple, rapid, accurate, and convenient in popularization.

Description

technical field [0001] The invention belongs to the application field of fluorescent nanometer materials, and in particular relates to a method for detecting residual clenbuterol in pork. Background technique [0002] Clenbuterol is a synthetic β-adrenergic stimulant, commonly known as "clenbuterol", which was originally used to treat bronchial asthma, especially chronic diseases. In the 1980s, American researchers discovered that high-dose use of clenbuterol can promote the growth of animal tissues, increase the percentage of lean meat and decompose fat. Subsequently, the drug was widely used in livestock and poultry production. However, later studies have shown that clenbuterol is very easy to accumulate in livestock and poultry. When people eat meat products with clenbuterol residues for a long time, adverse reactions such as headache, chest tightness, heart palpitations, and numbness of limbs will occur. Can be life threatening. Therefore, in order to ensure food safe...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6458G01N21/6486
Inventor 曹雪玲连丽丽王广红
Owner JILIN INST OF CHEM TECH