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A preparation method of engineered dental pulp

An engineering and endodontic technology, applied in the direction of biochemical equipment and methods, culture process, bone/connective tissue cells, etc., can solve the problem of not having natural tooth root morphology and structure

Active Publication Date: 2021-09-10
XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] American scholar Huang GT et al. combined fibrin glue with root-end dental papilla stem cells to form cell clusters, implanted them in the root cap and subcutaneously on the back of immunodeficient mice, and took out the implants 3 to 4 months later. A regenerated dentin layer, odontoblast layer, and vascularized pulp tissue were observed, but lacked the morphology of a native tooth root
There is no report on the establishment of engineered dental pulp for root reconstruction and regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Embodiment 1, one of the preparation methods of engineered dental pulp

[0034] Its preparation steps are as follows:

[0035] Step 1. Dental pulp cell culture: Mince the pulp tissue of deciduous teeth of miniature pigs, digest with 0.2g / L type Ⅰ collagenase and 0.1g / L Dispase enzyme for 1 hour until the tissue is loose, blow and beat the pulp with a straw The organization is fully dispersed. Sieve through a sieve to form a single cell suspension, discard the supernatant after centrifugation, then add dental pulp cell culture medium to resuspend the cells, transfer to a cell culture bottle, 37 ° C, 5% CO 2 Cultured in an incubator, the medium was changed every other day, and the cells were cultured to 90% confluence and then passaged to obtain the second generation of dental pulp cells.

[0036] The composition of the dental pulp cell culture medium A is: 10% (v / v) fetal bovine serum, 0.2 mg / mL glutamine, 75 ng / mL basic fibroblast growth factor in commercial α-MEM cul...

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Abstract

The invention relates to a preparation method of an engineered dental pulp, which is used for tooth root reconstruction. The dental pulp is composed of dental pulp cells and the extracellular matrix they secrete and synthesize. That is, the dental pulp cells are expanded and cultured to form stratified dental pulp cell aggregates, and then a plurality of dental pulp cell aggregates are formed. Laminates are placed and shaped to form engineered pulp. The dental pulp of the present invention can form a new dentin structure in vivo, so that the root of the tooth can continue to develop. The dental pulp has the advantages of simple preparation, strong plasticity, suitable cell density, rich extracellular matrix content, no need for scaffold materials, and short in vitro culture time.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a preparation method of engineered dental pulp for tooth root reconstruction and regeneration. Background technique [0002] After the tooth erupts, the tooth root relies on the living pulp cells in the root canal and periapical area to continue to develop and form a tooth root. It takes about 3 to 5 years for the tooth root to complete its development. When the root develops due to trauma, caries, or deformed central cusp, etc., the pulp infection and cell necrosis can stop the development of the root. For young permanent teeth with undeveloped roots, the length and thickness of the roots are insufficient, and the apical foramen is open. Such teeth cannot withstand normal chewing force, and the service life is shortened. Therefore, the young permanent teeth continue to develop and the apical foramen closes. , the recovery of the periapical tissue has impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077A61L27/38
CPCA61L27/3804A61L27/3865A61L2430/12C12N5/0664C12N2500/32C12N2500/38C12N2501/115C12N2501/15C12N2501/39C12N2501/999
Inventor 张勇杰
Owner XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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