Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating pig skin epidermis and dermis

A technology of dermis and epidermis, which is applied in the field of separation and extraction of pigskin skin epidermis and dermis, can solve the problems of different drug distribution and content, affecting test results, and other problems, achieving fewer influencing factors, improving reliability, and scientific methods Effect

Inactive Publication Date: 2017-06-27
湖北省疾病预防控制中心
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, the problem in the prior art is: the existing method for obtaining materials for the stratum corneum of the skin commonly used is that the adhesive tape method is only applicable to the medicines acting on the stratum corneum, and cannot be applied to acting on the deeper layers of the epidermis (such as the basal layer, spinous layer). , granular layer, transparent layer, etc.) and dermis, the pressure used for sampling is different, and the amount of stratum corneum attached to each sample is also different, that is to say, the thickness of the stratum corneum is different, and the distribution and content of the drug Also different, commonly used methods will affect the test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating pig skin epidermis and dermis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0022] Such as figure 1 As shown, the method for separating and sampling pigskin skin epidermis and dermis layer provided by the embodiments of the present invention comprises the following steps:

[0023] S101: separating the epidermis and dermis of the miniature pig from the skin from the skin muscle;

[0024] S102: The separated mini-pig skin is paved with cardboard to keep the skin flat, and the mini-pig skin is cut into squares of 1.0cm*2.0cm with a sharp scalpel in the shape of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of dermatologic medicine pharmacokinetics and discloses a method for separating pig skin epidermis and dermis. The method includes: using a microscope to precisely measure the thickness of the epidermis and the dermis, and using the frozen section technology to separate the epidermis and the dermis so as to achieve medicine skin hierarchical distribution researches. The method is scientific, effective, precise, free of pollution, quite significant to the preclinical pharmacokinetics researches of local external-use medicine, few in influence factors and simple to operate.

Description

technical field [0001] The invention belongs to the technical field of skin pharmacokinetics, and in particular relates to a method for separating the epidermis and dermis of pigskin. Background technique [0002] Skin pharmacokinetics is a science that studies the absorption, distribution, metabolism and elimination process of drugs and their metabolites in the local skin. It plays an extremely important guiding role in the development and evaluation of topical skin drugs. The lesions of different skin diseases involve different parts, some lesions only involve the epidermis, and some lesions not only involve the epidermis, but also go deep into the dermis. Therefore, for different indications, pharmacokinetic tests should be carried out according to the different parts of the lesion involved, to observe whether the drug can reach the lesion site, and to determine whether the drug concentration at the site is sufficient to exert its curative effect. The skin of minipigs is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/06
CPCG01N1/06
Inventor 余启枝郏自明黎炎梅杨文祥郑艳华王轶童瑶刘家发唐利军
Owner 湖北省疾病预防控制中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com